Application and evaluation of human antibodies in ABO grouping
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 10
Abstract
Objective To evaluate the application of monoclonal typing reagents and human anti-A/B antibodies for absorption-elution test in ABO grouping. Methods The specificity of monoclonal typing reagents and human anti-A/B antibodies with standard A, B, O and AB phenotypes at 4 ℃, room temperature, and 37 ℃ were compared. Affinity was evaluated by the titer, agglutination time and agglutination intensity of the reaction with A1/B cells. 29 samples with ABO discrepancy were tested to evaluate the ability of monoclonal typing reagents and human anti-A/B antibodies to detect weak antigens in absorption-elution test. Results The specificity and affinity of human anti-A/B antibodies are low, and monoclonal typing reagents have cross reactivity. Human anti-A/B antibodies can detect most weak antigens in absorption-elution test with no cross reactivity. Conclusion In ABO grouping, the human anti A/B antibody binding absorption-elution test can serve as a supplement method for identifying ABO weak antigens. Accurate results can be obtained with reasonable reagents and corresponding methodology in serological tests,thus ensuring the safety of blood transfusion.
Authors and Affiliations
Qinqin ZUO, Liangzi ZHANG, Dazhou WU, Yong ZHANG, Xiaoyue CHU, Weiwei ZHANG, Hong WANG, Juan MAO, Hua XU
Keywords
Related Articles
- EP ID EP738667
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.10.016
- Views 43
- Downloads 0
Preliminary study on HUVEC permeability changes in FNAIT induced by CD36 antibody
Objective To explore the pathogenesis of fetal edema caused by CD36 antibody in fetal/neonatal alloimmune thrombocytopenia (FNAIT), and to provide reference for clinical prevention and treatment. Methods The established...
Prevalence of HEV infection markers in Chinese blood donors: a meta-analysis
Objective To analyze the serological markers and RNA prevalence of HEV infection in Chinese voluntary blood donors in different regions of China, so as to provide basis for the necessity of HEV screening and the formulat...
Analysis on the characteristics of rural blood donation population in Dali Bai Autonomous Prefecture
Objective To explore the status and characteristics of voluntary blood donors in rural areas of Dali Bai Autonomous Prefecture (referred as Dali), and to provide basis for scientific and effective voluntary blood donatio...
Changes in serum immunoglobulin levels in children with thalassemia who undergo repeated blood transfusions and their correlation with delayed hemolytic transfusion reactions
Objective To study the changes in serum immunoglobulin levels in children with thalassemia who undergo repeated blood transfusions and explore their correlation with delayed hemolytic transfusion reactions(DHTR). Methods...
Efficacy of apheresis and concentrated platelets under different transfusion strategies
Objective To retrospectively analyze the clinical effects of apheresis and concentrated platelets under different transfusion strategies, so as to explore more scientific transfusion strategies. Methods A total of 279 pa...