Application of in-house nested polymerase chain reaction for rapid diagnosis of tuberculous pleural effusion
Journal Title: International Journal of Medical Research and Review - Year 2016, Vol 4, Issue 5
Abstract
Tuberculous pleural effusion (TPE) is a common manifestation of extrapulmonary tuberculosis and is the most common cause of pleural effusion in many countries. Conventional diagnostic tests for detection of Mycobacterium tuberculosis in pleural fluid or pleural tissue, have known limitations. Hence, there is need for a newer, rapid diagnostic tests. Molecular techniques, detecting DNA of M. tuberculosis in pleural fluid have better sensitivity and could be a potent tool for rapid diagnosis of tuberculous pleural effusion. Objective: To evaluate Nested PCR protocol targeting 38 kDa gene for rapid detection of M. tuberculosis complex in clinically suspected cases of TPE. Material and methods: A cross-sectional, prospective study was carried out at the tertiary care institute in a rural setup at western U.P. A total of 155 subjects with clinical suspicion of TPE enrolled during February 2015 to January 2016. About 10-20 ml of pleural fluid was collected and analysed for presence of M .tuberculosis by Z.N staining, culture on Lowenstein Medium (LJ), BacT/Alert 3D culture bottle and by Nested PCR targeting 38kDa gene of M. tuberculosis. Result: Off the 155 patients enrolled, M. tuberculosis was detected by AFB staining, LJ culture and BacT/Alert 3D system staining in 13 (8.4%), 45 (29%) and 72 (46.5%) respectively. Diagnostic sensitivity of nested PCR (nPCR) was 60.6% and among smear positive and culture negative samples, sensitivity was 100% while in smear negative, culture negative it was 29.2%. Conclusion: 38 kDa based nested PCR offers alternative robust approach for rapid and accurate detection of M .tuberculosis in paucibacillary tuberculous pleural effusion specimens.
Authors and Affiliations
Amit Singh, Adesh Kumar, Vineet Chaturvedi, Pradeep Sharma
Keywords
Related Articles
- EP ID EP230081
- DOI 10.17511
- Views 161
- Downloads 0
Knowledge and attitude of Al-Ghad college students towards corona virus infection
Objective: This study was conducted to identify the knowledge, attitudes and beliefs towards Corona virus infection among students in Al-Ghad International College for Applied Medical Science in Riyadh Male Campus. Metho...
Title: Hyperglycemia in critically ill patients and its association with increased mortality. A hospital based observational study
Objective: Hyperglycemia is an independent risk factor in critically ill patients, contributing to increased mortality and morbidity. This study was conducted to see the adverse effects of hyperglycemia in the form of in...
Assessment of heart rate variability and reaction time in traffic policemen
Introduction: Air Pollution can have a deleterious effect on cardiovascular function and cognition. Heart rate variability (HRV), which is a non-invasive and objective measure of cardiac autonomic function, is a cardiova...
Cadaver dissection study of the pectoral nerves for feasibility in brachial plexus injuries
Injury to upper trunk of Brachial Plexus is the most common injury pattern. Major functions affected those of supra scapular nerve (SSN), axillary nerve (AXN) and musculocutaneous nerve (MCN) which resulting dysfunction...
Role of bacterial vaginosis in preterm labor - a prospective study
Objective: To study the incidence of bacterial vaginosis in pregnant women in preterm labor and in labor at term and the relation of bacterial vaginosis with preterm delivery, neonatal birth weight and puerperal sepsis....