Are Bases Alkylated by Branched Alkylating Agents Removed by Nucleotide Excision Repair or Base Excision Repair?
Journal Title: Advances in Clinical Toxicology - Year 2022, Vol 7, Issue 2
Abstract
We have shown that comet-positive responses by adducts that are repaired by nucleotide excision repair (NER) and base excision repair (BER) are reduced by α-amanitin (AMN) and enhanced by 2’, 3’-dideoxythymidine (ddT), respectively. Based on this finding, bases alkylated by n-alkyl groups with <5 carbons and those with >4 carbons were discussed to be removed by BER and NER, respectively. To study whether bases alkylated by branched alkyl groups can be removed by NER or BER, 4 alkyl methanesulfonates that have a branched methyl group with different branching positions [iso-propyl methanesulfonates (iPMS), 1-methyl-pentyl methanesulfonate (1MPeMS), 2-methyl-pentyl methanesulfonate (2MPeMS), and 3-methyl-pentyl methanesulfonate (3MPeMS)] were synthesized. We studied the reduction of comet-positive responses by AMN and their enhancement by ddT for those alkyl methanesulfonates with a branched methyl group.
Authors and Affiliations
Kotaro Sasaki, Runa Kobayashi, Takanori Nakamura, Yasuaki Kikuchi, Galbreath Hannah Elizabeth, Katsutoshi Matsumoto, Akira Hasegawa, Takayuki Saito, Kumiko Sato, Tetsuo Honma, Ayumi Yamamoto, Hirotada Arai, Yoshihiro Kadoma, Satomi Kawaguchi, Kazuyuki Furuya, Marina Kobune and Yu F Sasaki*
Keywords
Related Articles
- EP ID EP751970
- DOI 10.23880/act-16000243
- Views 50
- Downloads 0
The Cause and Functions of Metal Oxide Nanoparticles for Toxicology Applications: A Review
Environmental impacts of metal oxide nanoparticles in toxicological research studies are rapidly spreading nowadays, many of which are exponentially increasing in various industrial and energy applications. Its usage are...
Nutritional Value and Associated Potentials
Seafood provides essential nutrients to the body. A study funded by CDC found that eating seafood for essential Omega-3 fatty acids can prevent 84,000 deaths each year. According to a Harvard study, 3-ounce servings of...
Sled Dogs as a Model for Brown Fat Research
Brown adipose tissue (BAT) has the unique function of burning energy to create heat through the process of non-shivering thermogenesis. Activation of BAT in humans as a means of combating obesity is an active area of re...
Safety Issues of Biosimilar Products
Biosimilars are not expected to be direct copies of biologic medicines and are therefore not the same as generic drugs. Due to the complex structure of biologic medicines and the processes involved in production, biosim...
Effect of Sodium Nitrate (NaNO3) on Sperm Motility and Abnormality: An In vitro Approach
Nitrates (NO3) are the naturally occurring inorganic ions with beneficial effects in both plants and animals. However, NO3 in excess has adverse effect on male and female reproductive systems. The study was undertaken to...