Assessment of genetic variation among Rosa species using ISSR genetic markers
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 7, Issue 3
Abstract
In this study, Inter-Simple Sequence Repeats (ISSR) markers were applied to assess genetic diversity and genetic relationships among seven species of Rosa. Six of ten used primers could amplify 81 scorable ISSR loci which 71 bands were polymorphic (86%). The 811 and 827 primers produced minimum and maximum bands, respectively. The percentage of polymorphic bands ranged from 60 to 100 with an average of 86.25 %. The size of amplified DNA fragments was ranging between 500 to 2500 bp which was used for calculation and statistical analysis. The cluster analysis based on the presence or absence of bands was performed by Jaccard’s similarity coefficient, based on Unweighted Pair Group Method with Arithmetic Averages (UPGMA). Similarity coefficient ranged from 0.30 to 0.66. Based on cluster analysis, the dendrogram revealed three main clusters. First cluster was divided into two subgroups. This investigation showed that genetic distance was relatively significant among these species. The results also propose that ISSR marker is a useful tool for evaluation of genetic diversity and relationships among different Rosa genotypes.
Authors and Affiliations
Leila Mirzaei, Fatemeh Rahmani, Mina Beigmohamadi
Keywords
Related Articles
- EP ID EP38628
- DOI -
- Views 148
- Downloads 0
Path coefficient analysis for the yield components of rice cultivars in Iran under different nitrogen levels
In order to determine the relationships among yield and yield components in rice (Oryza sativa L.), an experiment as factorial in RCBD with three replications was conducted during 2009 year in the Rice Research Institu...
Interleukin-1 beta response to an acute incremental cycling in obese men
To compare of circulating levels of IL-1b as an inflammation cytokine between obese and none obese men and also to investigation its response to an acute exercise test. Fasting blood samples were obtained of adult obese...
Evaluation of heavy metal concentration in the poultry feeds
The study was conducted in order to determine the heavy metals contamination in poultry feed. Twenty-one samples of poultry feed were collected from three feed companies and five poultry farms. The samples were analyze...
Diallel analysis of soluble protein in root nodules and seeds in blackgram [Vigna mungo (L.) Hepper]
The study was done to see the inheritance of soluble protein in two quantitative characters, such as seeds and nodules per plant in Blackgram [Vigna mungo (L.) Hepper]. Dominance and additivity were detected for soluble...
Leaf flavonoids of Convolvulus L. species in Markazi Province, Iran
In order to study the leaf flavonoids pattern of Convolvulus leaves in 12 populations from four species (C. arvensis, C. commutatus, C. lineatus and C. pilosellaefolius) originated from Markazi Province, Iran, twodimens...