Assessment of MicrobialContamination of Gutta-Percha Cones after opening a SealedPackage
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2019, Vol 18, Issue 2
Abstract
The goals of endodontic treatment are the proper access cavity preparation, cleaning, and shaping, and filling the root canal system,to prevent the microbial reinfection. Thus any material placed in the root canal must be free of microbial contamination to avoid root canal recontamination.Currently, gutta-percha is the most commonly used root canal filling material.Guttapercha is supplied in a sterilised sealed package. Only few cones maybe used for a patient and then the box is used again for another patient. Once the seal is opened, the Guttapercha cones are exposed to the environment and eventually lose their sterility. The aim of this study was to assess the sterility of the guttapercha cones in a sealed manufacturer’s supplied package.Two different sealed manufactures supplied package ofguttapercha cones namely, Dentsply and Coltene of 25sizewere opened under aseptic laboratory conditions. Five guttapercha cones from each packet were cultured in a nutrient agar plate incubated at 37 0 C and microbial colonies were counted.There was nooccuranceof microbial growth or contamination in the culture media on the first and second day after opening the sealed package but on the third day, growth of microorganism was detected in the culture media .Sealedpackages of guttapercha, supplied by the manufacturer are usually sterile and possible contamination of the cones mayoccur due to incorrect handling of the cones, leading to breaking of the aseptic chain.
Authors and Affiliations
Neingutunuo Angami, Pallavi Yaduka, Rubi Kataki, K Pewezo Khalo
Keywords
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- EP ID EP467747
- DOI 10.9790/0853-1802145861.
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