ASSESSMENT OF PROLIFERATIVE POTENTIAL OF TUMOR CELLS USING KI-67 EXPRESSION AND MORPHOMETRICAL ANALYSIS FOR PROGNOSTICATION OF ORAL SQUAMOUS CELL CARCINOMAS

Abstract

Background: Cancer cells exhibit a characteristic intrinsic ability of uncontrolled cell proliferation which plays a vital role in tumor development and progression. The Ki-67 antigen expression is strictly associated with cell proliferation which detects the cells entering the cell cycle. In addition, computer aided image analysis provides an objective and highly reproducible histological evaluation of OSCC. Assessment of Ki-67 expression along with morphometry may help in early and precise diagnosis and prognostication of OSCC. Aims: To correlate the ki-67 expression and the morphometrical parameters of oral squamous cell carcinomas and to assess the efficacy of the correlation in OSCC prognostication. Methods and material: OSCC cases (n=105) were examined immuno histochemically using Ki-67 antigen The nuclear area (NA), cell area (CA), nuclear perimeter (NP) and cell perimeter (CP) assessed using “image J 1.34 software”. The data was statistically analyzed. Results: Highly significant correlation was found between Ki-67 expression and the advancing grades of OSCC (p<.0001). NA (p=0.025), CA (p<.0001), NP (p=.027) and CP (p<.0001) of poorly differentiated OSCC were significantly lower than well differentiated and moderately differentiated OSCC. Follow up analysis revealed nuclear area and cell area to be higher in the recurrent cases than the non recurrent ones. Conclusion: Assessment of Ki- 67 expression and morphometry help in early and precise diagnosis and prognostication of OSCC. A correlation between Ki-67 expression and morphometrical analysis could not be ascertained in this study. Further studies with a larger sample number may provide more definitive results.

Authors and Affiliations

Rakheja Mahima| Post graduate Student, Department of Oral Pathology and Microbiology, Manipal College of Dental Sciences Manipal University, Manipal, Singh Pratyush| Post graduate Student, Department of Oral Pathology and Microbiology, Manipal College of Dental Sciences Manipal University, Manipal, Shergill Ankur K| Assistant Professor, Department of Oral Pathology and Microbiology, Manipal College of Dental Sciences Manipal University, Manipal, Guddattu Vasudeva| Senior Lecturer, Department of Biostatistics, Manipal University, Manipal, Solomon Monica C| Professor and Head, Department of Oral Pathology and Microbiology, Manipal College of Dental Sciences Manipal University, Manipal, Email: solomonmc@gmail.com

Keywords

Related Articles

STUDY ON ASSOCIATION OF SERUM HOMOCYSTEINE WITH CORONARY HEART DISEASE IN RURAL POPULATION

Background: CAD is the major cause of death. Many factors are responsible for causing CAD, but in 5 to 10 percent of CAD patients have none of the known risk factors. Risk factor modification is an integral part of the...

Relationship between Emotional Awareness and Social Creativity in Primary School Students

The present research reviews the relationship between emotional awareness and social creativity. The participants of this research were 234 (120 girls and 114 boys) of the primary school students of the four districts o...

The Impact of Educational Furniture of Schools on Learning and Academic Achievement of Students at Elementary Level

Accessing the summits of science and culture and further luster of every country in the scientific fields is rooted in education and training of that country, and supplying appropriate and efficient educational spaces,...

Comparison of IL-13 and IL-27 levels between schizophrenics and healthy subjects before and after antipsychotic administration

Many new evidences suggest cytokine activities involvement in pathogenicity of schizophrenia. Therefore, the aim of this study was to assess the serum levels of IL-13 and 27 in schizophrenics, and then compares them wit...

Investigating the Relationship of Organizational Commitment and Clinical Competence (Case study: Nurses Working in Montazeri Hospital, City of Najafabad, Iran, 2015)

Human resources committed to the organization not only reduces absence, delay, and replacement, but also causes the increase of organizational performance, employees’ mental freshness, better attainment to organizatio...

Download PDF file
  • EP ID EP11523
  • DOI 10.5958/2319-5886.2015.00163.0
  • Views 293
  • Downloads 9

How To Cite

Rakheja Mahima, Singh Pratyush, Shergill Ankur K, Guddattu Vasudeva, Solomon Monica C (2015). ASSESSMENT OF PROLIFERATIVE POTENTIAL OF TUMOR CELLS USING KI-67 EXPRESSION AND MORPHOMETRICAL ANALYSIS FOR PROGNOSTICATION OF ORAL SQUAMOUS CELL CARCINOMAS. International Journal of Medical Research & Health Sciences (IJMRHS), 4(4), 820-826. https://europub.co.uk./articles/-A-11523