Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth
Journal Title: Journal of Applied Veterinary Sciences - Year 2016, Vol 1, Issue 1
Abstract
This work presents cultural characterization of Peste Des Petites Ruminants (PPR) virus in VERO-culture. The VERO cells are currently considered as an acceptable cell substrate to produce a wide range of viruses. This study evaluates the best time for inoculation of PPR virus on VERO cell cultures; the study proved that the optimum time was 24 hours after subculture of VERO cell line using MOI 2: 1. It was also found that the best time of harvestation of virus fluid of PPR was 9th day post-inoculation to reach the best titre 6 log10 TCID50 /ml.
Authors and Affiliations
El-Dakhly A. T. , Youssef M. M. , Abeer A. Tammam, Namaa A. Mohamed
Keywords
Related Articles
- EP ID EP677144
- DOI 10.21608/JAVS.2016.62123
- Views 305
- Downloads 1
A High Light On Lumpy Skin Disease In Iraq And The Middle East: A Review Article
Lumpy skin disease is an infectious, eruptive disease that affected the different animal species, especially cattle . The causing virus is a member of the poxviridae family with Neethling strain. Transmission of the dise...
Role Of Taurine In Male Reproductive System Performance In Adult Male Rats Exposed To Oxiative Stress By Hydrogen Peroxide
The experiment has been conducted to study the role of taurine of the concentration (0.5 & 1%) in the performance of the male reproductive system of rats that are exposed to oxidative stress by hydrogen peroxide for six...
The effects of Gastro-intestinal Parasites on haemato-biochemical parameters of sheep in Kirkuk province, Iraq
The present study is intended to detect internal parasites in faecal samples of local sheep in Kirkuk province, Iraq, through the period between March and the end of November 2022. where, 130 blood and faecal samples wer...
Retrospective Study of Canine Pathologies Causing Mortalities in Ilorin Kwara State, Nigeria
Canine pathologies refer to the various diseases, disorders, and abnormalities that can affect a dogâs different body systems and cause mortality. The primary aim of this study was to investigate the common pathologies...
Toxicopathological Impacts Of Chlorpyrifos On Sperm Qualities And Testicular Tissue Alterations And Their Modulation With Vitamin E And Zinc In Male Albino Rats
This study was carried out on 30 mature male albino rats (2 months old, weighed about 130 g) in 3 groups of ten rats for each one in ARC. Group I, kept as control. Group II, daily intubated with CPF, at a dose level of 1...