Assisted selection in the improvement of soy for human consumption in the State of Tocantins
Journal Title: Revista Verde de Agroecologia e Desenvolvimento Sustentável - Year 2017, Vol 12, Issue 3
Abstract
Soy in human food has several health benefits, but finds resistance to its consumption by the western ones due to the flavor caused by the lipoxygenases enzymes. The objective of this study was to select soybean TN (Triple Null) hybrids in a population of the fourth self-fertilization cycle (F4) using techniques that use molecular markers. The experiment was conducted in the experimental area of the Federal University of Tocantins Campus Gurupi - TO. Hybridizations between soybean progenitors adapted to Tocantins and TN were performed. Obtained the F1 population in the greenhouse, F2, F3 and F4 were planted in the field, in the F4 generation was selection of superior hybrid for DNA analysis in order to identify hybrids with the trait complete absence of Lox2 and 3. We selected one hundred seventy-one hybrid, made up of DNA extraction and PCR of all. It was concluded that the highest percentage of TN hybrids was obtained at the intersection BRSMG 790A (TN) X GB874. Lox2 and Lox3 amplification primers confirmed as an essential tool in a soybean breeding program for human consumption. We selected 34 TN hybrids with the desired agronomic characteristics.
Authors and Affiliations
Aristoteles Capone, Alex Sandro Dario, Raimundo Wagner de Souza Aguiar, Rodrigo Ribeiro Fidelis, Hélio Bandeira Barros
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