Association between HLA-A, HLA-C and HLA-B Genes and Ankylosing Spondylitis in Macedonian Population
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2012, Vol 5, Issue 3
Abstract
Aim: The aim of this study was to determine the association of HLA-A, -C and -B genes with ankylosing spondylitis in patients from the Republic of Macedonia. Material and Methods: This study included 307 subjects (250 healthy individuals and 57 patients with ankylosing spondylitis who were diagnosed at the University Clinic of Rheumatology in Skopje). The HLA typing of class 1 (HLA-A, HLA-C and HLA-B) genes was performed using the method of Reverse Line Strip, after isolation of DNK from the blood leucocytes with the standard phenol-chloroforme method. The HLA sub typing of HLA-B*27 was performed with high resolution single-strand polymorphism. Results: We found negative association between HLA-A*03, HLA-A*24, HLA-C*04, and HLA-B*18, as well as positive association between HLA-C*01, HLA-C*02, and HLA-B*27 group specific alleles and patients with ankylosing spondylitis. Both HLA-B*27:02 and HLA-B*27:05 alleles have shown positive association with ankylosing spondylitis. Conclusion: Group specific alleles HLA-A*03, HLA-A*24, HLA-C*04, and HLA-B*18 are significantly more frequent in healthy individuals than in patients with ankylosing spondylitis which suggests their protective role. HLA-C*01, HLA-C*02, and HLA-B*27 group specific alleles (HLA-B*27:02 and HLAB*27:05) have strong positive or susceptible association with ankylosing spondylitis.
Authors and Affiliations
Meri Kirijas| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia, Snezhana Mishevska-Perchinkova| University Clinic of Rheumatology, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia, Anzelika Karadzova-Stojanoska| University Clinic of Rheumatology, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia, Olivija Efinska-Mladenovska| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia, Aleksandar Petlichkovski| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia, Dejan Trajkov| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia, Mirko Spiroski| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia
Keywords
Related Articles
- EP ID EP8871
- DOI 10.3889/MJMS.1857-5773.2012.0255
- Views 353
- Downloads 17
Quantitative Analysis of Adrenal Cortical Histological Alterations After Application of Medroxyprogesterone Acetate
Aim. The aim of our investigation was to make a quantification of the changes being registered by a quantitative histological analysis of adrenal cortex after long-term application of high doses of medroxyprogesterone...
Macedonian Biomedical Scientists Are Not Adequately Represented in BiomedExperts Database
Aim. The aim of this study was to analyze publication activity of Macedonian biomedical scientists reported in BioMedExperts and to propose improvements and corrections in order to present Macedonian science more objec...
Changes in Some Pharmacokinetics Parameters of Chloroquine by Gnetum Africana
Effect of Gnetum africana on the pharmacokinetic parameters of chloroquine was investigated. Chloroquine phosphate (200 mg/Kg) was concurrently administered to overnight fasted albino rats. Blood samples were collected...
Hydatid Disease with Multiple Organ Involvement: A Case Report
We present a case of 34 years old woman with hydatid disease of the lungs, brain and heart but without liver involvement. The disease started in 2006. as pulmonary hydatidosis of the right posterior inferior lobe. In J...
Synovial Sarcoma of the Liver – A Case Report
We report a case of synovial sarcoma of liver in a 44 year old man, presented as a tumor mass in left hepatic lobe. The patient was admitted at the hospital with clinical symptoms of acute abdomen and severe pain in th...