Association of Apa I polymorphism of vitamin d receptor gene with type 2 diabetes mellitus in Saudi population
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
Vitamin D deficiency has been associated with the acceleration of the onset of T2DM. The biological effects of vitamin D are mediated by binding to the vitamin D receptor (VDR) which belongs to the steroid receptor superfamily. Although many polymorphisms exist in the VDR gene, their effect on VDR protein function and signalling is unknown. An association between VDR polymorphism and T2DM has been reported in some studies; however, it appears to vary across different populations around the world. Hence this study was carried out to investigate the relationship between VDR gene polymorphisms at three restriction sites ApaI, BsmI, and TaqI and the risk of T2DM in Saudi population. The volunteers were classified according to Fasting Blood Glucose (FBG) test as two groups, T2DM and normal group. The VDR gene was amplified by polymerase chain reaction (PCR). PCR products were digested using restriction enzymes: ApaI, TaqI, and BsmI and the bands were visualised by agarose gel electrophoresis and ethidium bromide dye under UV. The results of the current study showed a significant difference in genotypes and allele frequencies of the VDR gene polymorphisms at the ApaI site between T2DM patients and control groups.
Authors and Affiliations
Archana P. Iyer, Susan Lanham New, Sawsan Khoja, Maryam A. AL-Ghamdi, Sami Bahlas
Keywords
Related Articles
- EP ID EP428064
- DOI 10.18006/2017.5(2).271.276
- Views 69
- Downloads 0
EFFICACY OF ATRAZINE HERBICIDE FOR MAIZE WEED CONTROL IN NEW ALLUVIAL ZONE OF WEST BENGAL
Field study was conducted in during 2015 and 2016 to evaluate the efficacy of new formulation of atrazine herbicide on weed control in maize. The experiment was conducted at Research farm, kalyani of BCKV. The treatment...
Assessment of the antioxidant properties of the most common coffee brews available in the local markets of the western region of Saudi Arabia
The purpose of the current study is to determine the antioxidant activity of the most commonly used coffee brews in the Western region of Saudi Arabia. Further, total phenolic and flavonoid contents were measured and ant...
SCANNING ELECTRON MICROSCOPIC STUDY ON POLLENS OF EIGHT BEE FLORAL RESOURCES FROM HAMIRPUR HILLS, HIMACHAL PRADESH, INDIA
Scanning electron microscopy study on pollens of eight important bee floral resources collected from Hamirpur hills of Himachal Pradesh were carried. Pollens grains were investigated for size, shape, types of pores, exin...
Estimation of evapotranspiration for basilicata region with a pennman-monteith method
Estimation of reference evapotranspiration rate is a very significant parameter in agriculture science. It indicates the water consumption of the plant. So, it plays an important role in irrigation scheduling as well as...
Comparative study of two rice cultivars (Oryza glaberrima and O. Sativa) under different cultural conditions
This study was conducted to evaluate and compare the growth performance of two rice species viz Oryza glaberrima and O. sativa under different hydrological conditions. For this, 21 accessions of O. glaberrima and 69 of O...