Automated system for counting and sorting cells in sugarcane suspensions
Journal Title: Biotecnología Vegetal - Year 2003, Vol 3, Issue 1
Abstract
The quantity and types (embryogenic and non-embryogenic) of cells are very important parameters to evaluate plant cell suspension growth for determine the best suspension to obtaining plant regeneration by somatic embryogenesis. These parameters are determined normally by visual human inspection in the microscope. It provokes fatigue and time lost to biologist and non-precise results. By solve these problems an automatic system was implemented. A CCD-camera is attached at the microscope and a 256 grey level image is digitised. The cell clusters segmentation and position in the image are calculated using the competitive learning and contour tracking technique. The cell cluster with a little starch content are counting and classify by the topological and directional information extracted of cell cluster skeleton. The cell clusters with high starch content are counting and classify by the critical point of cluster contour. The system was validate in Sugar-cane suspension (Saccharum sp.). var. CP 52-43. The final result obtained by human visual inspection and by the automatic system implemented by us was found similar.
Authors and Affiliations
Evelio Báez Pérez
Keywords
Related Articles
- EP ID EP697874
- DOI -
- Views 57
- Downloads 0
In vitro crop physiology
The culture procedures described up to the eighties, did not made any mention to the environmental control of in vitro plant development. However, growth rate, development and many of the physiologic-morphologic features...
Effect of immersion time on in vitro multiplication of Bambusa vulgaris Schrader ex Wendland in RITA® TIS
The TIS provides solutions to the constraints that affecting in vitro propagation of bamboos and increases the quality of the plants in vitro propagated and survival of these in greenhouse conditions and field. This stu...
Automated system for counting and sorting cells in sugarcane suspensions
The quantity and types (embryogenic and non-embryogenic) of cells are very important parameters to evaluate plant cell suspension growth for determine the best suspension to obtaining plant regeneration by somatic embryo...
Management of suckers and apices of cultivars of Musa spp. to initiate micropropagation and behavior during six in vitro subcultures
Two forms of handling of the tillers were evaluated to extract the apexes that will be established in vitro. One was the method of the planting the rhizomes which below to the small tiller in the concrete beds, to cause...
Hydrophilic compounds in culture filtrates of Fusarium oxysporum f. sp. cubense GCV [01210] induce protection to banana leave toward a main pathogen phytotoxic component
Panama disease caused by Fusarium oxysporum f. sp. cubense (Foc), is among the most important diseases in Musa spp. Foc is a necrotrophic fungus, their phytotoxins play a role in disease development. Previously culture...