Bacteriological Profile of Osteomyelitis in a Tertiary Care Hospital
Journal Title: International Journal of Medical Science and Innovative Research (IJMSIR) - Year 2019, Vol 4, Issue 8
Abstract
Background: Widespread use of antibiotics has changed the etiological pattern and antibiotic susceptibility pattern of Osteomyelitis. Continuous monitoring in a particular geographical area will indicate the current resistance patterns, which would help in the initiation of appropriate prophylactic antibiotic until the culture reports, are available. Objectives: 1. To identify Aerobic bacteria isolated from pus and tissue samples of patients suspected with Osteomyelitis. 2. To study the Antibiotic susceptibility pattern of the isolates. Methods: 104 cases clinically diagnosed as Osteomyelitis were included in the study. Aspirated pus / swabs or tissue samples were collected from the patients maintaining strict asepsis. Gram’s stain was performed from the samples and inoculated onto MacConkey Agar, Blood Agar, Nutrient agar and incubated aerobically at 370 C for 18-24hours and was observed for growth. The bacterial colony isolated was identified by colony morphology, cultural characteristics and biochemical reactions according to the standard techniques. Antibiotic sensitivity testing was performed according to CLSI guidelines by Kirby – Bauer disk diffusion method in Mueller Hinton agar plates. Results: Staphylococcus aureus (32%) was the most common organism isolated followed by Pseudomonas aeruginosa(15.6%), Citrobacter freundii (11.5%), Proteus mirabilis and Klebsiella pneumoniae (9%each), Escherichia coli(5.7%), Acinetobacter baumannii(4.9%),Citrobacter koseri(4.1%), Coagulase negative Staphylococci (3.3%), Enterobacter aerogenes (2.5%), Enterococcus faecalis (1.6%), Proteus vulgaris (0.8%). For Staphylococcus aureus , Vancomycin (100%), Teicoplanin (100%), Linezolid (97.4%) and Doxycycline (79.5%) were the most sensitive antibiotics .For the Gram negative bacilli (Fermenters ) , the most sensitive antibiotics were – Cefoxitin (76.7%) and Imipenem(65.4%).Amongst the Gram negative bacilli ( Non fermenters ) – Tigecycline ( 100%) , Meropenem , Aztreonam and Amikacin (68% each) were the most sensitive antibiotics. Conclusion: Proper selection of an antibiotic preceded by a bacterial culture and sensitivity is very important to prevent emergence of drug resistance in an organism and decrease indiscriminate use of unnecessary antibiotics.
Authors and Affiliations
Naik Shalini Ashok
Keywords
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