Bacteriological studies on bacterial pathogens isolated from broiler chickens with swollen head syndrome
Journal Title: Journal of Veterinary Medical Research - Year 2018, Vol 25, Issue 1
Abstract
This work was planned to investigate the bacteria isolated from broiler chickens head suffered from naked eye pathological lesions. Out of 200 examined head lesions, the result revealed that the major pathogens associated with swollen head syndrome (SHS) were Escherichia coli, Streptococcus dysgalactiae and Pseudomonas aeruginosa. Antimicrobial sensitivity pattern against 11 different antimicrobials proved that isolates were resistant to most of the tested antimicrobial agents. PCR was applied on 4 MDR E. coli, 4 S. dysgalactiae and 2 P. aeruginosa for detection of some resistance and virulence genes. The results of E. coli isolates revealed that blaTEM gene was the most prevalent in all isolates (100%) followed by tetA (A), aada1, aada2 and aacC genes. Meanwhile tetA (B) gene was found in 3 (75%), while aadB gene was not detected in any isolates. All S. dysgalactiae proved to harbour 16srRNAgene also all S. dysgalactiae were 100% positive for tuf gene followed by speF gene which found in 2 isolate (50%). The results of PCR of P. aeruginosa isolates revealed that toxA gene was the most prevalent gene found in all isolates (100%) followed by lasI. Then, phzM gene was found in one isolate (50%).
Authors and Affiliations
Ismail Abd El-Hafeez Radwan, Mohamed Fathy Mohamed, Aya Kamal Eldin Ahmed
Chemical and bacteriological evaluation of some crustaceans
Forty samples of crustaceans, 20 shrimp (local, imported, peeled and nonpeeled) and 20 local chilled samples were collected from Beni-Suef markets. Samples were evaluated by physiochemical deteriorative criteria (pH, TVB...
Efficacy of Ultra-sonographic Examination and Combined Use of PGf2α plus Cephapirin to Identify and Treat Endometritis in Dairy Cows
The objectives of the present study were to validate ultra-sonographic examination (US) as a reliable diagnostic tool for endometritis, as well as to determine the effects of intrauterine infusion (IU) of benzathine ceph...
Serogrouping and resistance gene detection in avian pathogenic E.coli isolated from broiler chickens
E coli infection in poultry is one of the principle causes of mortality and morbidity in chickens and turkeys resulting in retardation of growth and decreased feed conversion rate . The most common form of collibacillosi...
Comparative pharmacokinetic and renal clearance study of ceftiofur in cross breed Friesian and Buffalo calves
The pharmacokinetic profile of ceftiofur sodium, a third generation cephalosporin, was studied in both Friesian and buffalo calves following a single intravenous and intramuscular administration of 2.2 mg kg-1 b.wt. in a...
Characterization of antimicrobial resistant bacterial pathogens recovered from cases of bovine mastitis with special reference to Staphylococcus aureus
In the current study, a total of 20 and 78 milk samples were collected from animals showed signs of clinical and subclinical mastitis, for isolation and identification of different causative pathogens in some dairy farms...