Bio-incidence of bovine Johne’s disease in dairy buffaloes in Central and North India using sensitive goat based ‘indigenous elisa kit’ and traditional tests
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2016, Vol 4, Issue 0
Abstract
Present study was aimed to estimate the bio-incidence of Bovine Johne’s disease in native population of buffaloes (Murrah, Zaffarabadi and Bhadawari) in dairy farms and farmer’s herds located in Central and North India. Total 156 serum samples were screened from a dairy herd in Kiratpur (Malwa region), and 41.0% buffaloes were positive for Mycobacterium avium subsp. paratuberculosis (MAP) infection using ‘Indigenous ELISA kit’. In another study, 101 young males (farmer’s herds) from the native tract (Jind / Rohtak, Haryana) of Murrah breed were screened and 80.1% were reported positive by kit. In a farm herd of Bhadawari buffaloes in its native tract (Etawah district in Uttar Pradesh) and dairy herd of Murrah buffaloes procured from native tract and kept in Mathura district of Uttar Pradesh, In the two farms 20.0% buffaloes were screened and 71.4% were found positive by fecal Microscopy. Study reported high bio-incidence of BJD in native breeds of buffaloes in Malwa region of Central India, Etawah and Mathura (Uttar Pradesh) and Jind / Rohtak (Haryana) regions of North India, despite high slaughter rate, for meat harvesting. Goat based 'Indigenous ELISA kit' was employed for screening since no commercial kits were costly for the screening of buffaloes. Moreover Imported 'ELISA kits' were based on PPA from 'Cattle Type' biotype', a different strain from other geographical regions / country. ‘Indigenous ELISA kit’ using native 'Indian Bison type' biotype was efficient, specific and cost effective 'mass screening' test to estimate bio-incidence of BJD in large population of buffaloes at National level.
Authors and Affiliations
Sachin Digambar Audarya, Daljeet Chhabra, Sahzad . , Manju Singh, Bjorn John Stephen, Kundan Kumar Chaubey, Saurabh Gupta, Jagdip Singh Sohal, Kuldeep Dhama, Shivalingappa Yamanappa Mukartal, Doddamane Rathnamma, Shoor Vir Singh
Optimizing yield and fiber quality of cotton under mediterranean environment: managing nitrogen and potassium nutrition
Effective management strategies for nitrogen (N) and potassium (K) fertilizer are important to ensure optimum yield and fiber quality of cotton production. The aim of this research was to study the influence of nitrogen...
QUANTIFICATION OF Staphylococcus aureus AND Escherichia coli FROM BOVINE SUBCLINICAL MILK SAMPLES BY CONVENTIONAL PCR
The present study explores the application of conventional PCR and Mole v/s Avogadro’s number using single copy genes of Staphylococcus aureus and Escherichia coli for assessing bacterial load in milk samples from mastit...
STUDYING INDICATORS OF GELATIN AND NATURAL POLYSACCHARIDES QUALITY AND SAFETY
Present study was conducted with the aim of finding out the optimal composition of biodegradable packaging materials based on gelatin and natural polysaccharides, film samples. The film samples have been obtained with th...
Genetic variability for grain quality traits in indigenous rice landraces of Chhattisgarh India
The investigation was carried out to estimate the components of genetic variability and associated statistical parameters for grain quality traits of 215 indigenous rice landraces of Chhattisgarh, India. Substantial gene...
INFLUENCE OF ORGANIC MANURE, ON MICROBIOLOGICAL PROPERTIES OF CALCAREOUS SOIL
A long-term field experiment was started during rabi 1988-89 in calcareous soil at RAU, Pusa Farm in split plot design with NPK levels in main plots and organic sources in sub-plots. Rice and wheat as 35th and 36th crop...