Biochemical Identification of Aggregatibacter actinomycetemcomitans in an Indian Sample with Aggressive Periodontitis
Journal Title: Journal of Advances in Microbiology - Year 2017, Vol 7, Issue 1
Abstract
Background: The role of microorganisms and the etiology of periodontal disease is well established. Despite the fact that periodontal diseases are caused by dental plaque there existed some controversies regarding the role of specific organisms in the pathogenesis of the periodontal disease. This problem was overcome by the specific plaque hypothesis. Generally, the periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa) is identified employing anaerobic culture and in recent years through polymerized chain reaction. Very few attempts have been done to establish the confirmation of Aa through biochemical reactions. Hence this study was under taken to confirm Aa from the plaque sample by various biochemical tests. Aim of the Study: Biochemical identification of Aggregatibacter actinomycetemcomitans, a specific periodontal pathogen from the plaque samples of aggressive periodontitis patients. Materials and Methods: A total of forty patients 24 males and 16 females in the age range of 18-25 yrs. Diagnosed as aggressive periodontitis were included in this study. Plaque samples were collected from the periodontal pockets of aggressive periodontitis patients and were subjected to various biochemical tests. Results: Biochemical tests confirmed that the periodontal pathogen collected from the plaque samples were Aggregatibacter actinomycetemcomitans. Conclusion: Biochemical tests can be used as a viable economic alternative for the confirmation of the periodontal pathogen Aa.
Authors and Affiliations
R. Saranyan, B. Manovijay, G. Balaji Babu, Rajasekar . , I. Nithya, A. Anitha
Keywords
Related Articles
- EP ID EP338277
- DOI 10.9734/JAMB/2017/37877
- Views 95
- Downloads 0
Optimizing C:N Ratio, C:P Ratio, and pH for Biosurfactant Production by Pseudomonas fluorescens
Aim: To optimize the combination of selected culture medium parameters so as to achieve maximum biosurfactant production from Pseudomonas fluorescens. Study Design: (1) Culturing P. fluorescens for biosurfactant producti...
Phytochemical and Antimicrobial Activity Screening of Ether Extract of Afromomum melegueta Seed
Aim: The search for new antimicrobials as a result of antibiotic failure has led to the development of new antibiotic scaffolds. In the African sub-continent, this has translated to exploration of her rich flora for pote...
Influence of Bradyrhizobium and Mycorrhiza on Growth, Yield and Phosphorus Use Efficiency on Soybean under Manure Application
A field experiment was conducted at the Federal University of Agriculture, Abeokuta, South West Nigeria, to determine the influence of Bradyrhizobium and mycorrhiza on growth, yield and phosphorus use efficiency of soybe...
Occurrence of Bacterial Contaminants in Fin-fishes (Clarias gariepinus and Coptodon guineensis) from Humic Freshwater Ecosystem of Eniong River, Itu, Akwa Ibom State, Nigeria
The occurrence of bacterial isolates in fin-fishes (Clarias gariepinus and Coptodon guineensis) from the humic ecosystem of Eniong River, Akwa Ibom State was investigated. The results obtained from pour plate analysis sh...
Multidrug Resistance, V16S rRNA Gene Sequencing, High Frequency of Toxins Encoding Genes (tox, tdh, trh) in Parahaemolyticus and Non -parahaemolyticus Vibrios Isolated from Shellfish
PCR amplification of V16S-rRNA, tox, tdh, trh was applied as a molecular typing approach to identify potentially toxigenic vibrio species. Thirty vibrio strains were isolated from several types of shellfish collected fro...