Biochemical Identification of Aggregatibacter actinomycetemcomitans in an Indian Sample with Aggressive Periodontitis
Journal Title: Journal of Advances in Microbiology - Year 2017, Vol 7, Issue 1
Abstract
Background: The role of microorganisms and the etiology of periodontal disease is well established. Despite the fact that periodontal diseases are caused by dental plaque there existed some controversies regarding the role of specific organisms in the pathogenesis of the periodontal disease. This problem was overcome by the specific plaque hypothesis. Generally, the periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa) is identified employing anaerobic culture and in recent years through polymerized chain reaction. Very few attempts have been done to establish the confirmation of Aa through biochemical reactions. Hence this study was under taken to confirm Aa from the plaque sample by various biochemical tests. Aim of the Study: Biochemical identification of Aggregatibacter actinomycetemcomitans, a specific periodontal pathogen from the plaque samples of aggressive periodontitis patients. Materials and Methods: A total of forty patients 24 males and 16 females in the age range of 18-25 yrs. Diagnosed as aggressive periodontitis were included in this study. Plaque samples were collected from the periodontal pockets of aggressive periodontitis patients and were subjected to various biochemical tests. Results: Biochemical tests confirmed that the periodontal pathogen collected from the plaque samples were Aggregatibacter actinomycetemcomitans. Conclusion: Biochemical tests can be used as a viable economic alternative for the confirmation of the periodontal pathogen Aa.
Authors and Affiliations
R. Saranyan, B. Manovijay, G. Balaji Babu, Rajasekar . , I. Nithya, A. Anitha
Keywords
Related Articles
- EP ID EP338277
- DOI 10.9734/JAMB/2017/37877
- Views 106
- Downloads 0
Seroprevalence of Hepatitis B and C Viruses among Human Immunodeficiency Virus Infected Patients Accessing Healthcare in Federal Medical Centre, Keffi, Nigeria
Infections of Hepatitis B and C viruses among seropositive Human immunodeficiency virus patients are a growing public health problem in Sub Saharan Africa characterized by unaffordable treatment, severe morbidity and ass...
Prevalence of Listeria monocytogenes in Fresh and Raw Fish, Chicken and Beef
There has been recent outbreaks of food poisoning associated with Listeria monocytogenes. Most of the outbreaks were attributed to fresh meat and dairy products, and processed foods. This study was therefore carried out...
Antibiotics Susceptibility Patterns and Plasmid Profile of Staphylococcus aureus Isolated from Patients with Wound Infections Attending Four Hospitals in Akure, Ondo State
Aims: Several studies have documented Staphylococcus aureus as a leading pathogen implicated in wound infections with a remarkable potential for antibiotics resistance. This study determined the susceptibility patterns o...
Multidrug Resistance, V16S rRNA Gene Sequencing, High Frequency of Toxins Encoding Genes (tox, tdh, trh) in Parahaemolyticus and Non -parahaemolyticus Vibrios Isolated from Shellfish
PCR amplification of V16S-rRNA, tox, tdh, trh was applied as a molecular typing approach to identify potentially toxigenic vibrio species. Thirty vibrio strains were isolated from several types of shellfish collected fro...
Effect of Alkaline Treatment on Nutrient and Antinutrient Contents of Mucuna pruriens (L.) DC.
Aims: To investigate the impact of Trona and woodash (alkaline tenderizers) on aniti-nutrient and nutrient content of Mucuna pruriens during fermentation. Study Design: Natural fermentation and fermentation using additiv...