BIODEGRADATION OF CRUDE OIL AND LIGNIN-MODIFYING ENZYME ACTIVITY OF WHITE ROT BASIDIOMYCETES
Journal Title: Ecological Engineering and Environment Protection (EEEP) - Year 2019, Vol 1, Issue 1
Abstract
In the present study, the ability of eighteen white-rot basidiomycetes was evaluated for crude oil biodegradation. Cerrena unicolor strains, Panus tigrinus 433, P. lecometei 903, Pleurotus ostreatus 70, Trametes maxima 403, and T. versicolor 159 showed especially abundant mycelial growth on the surface of agar covered with droplets of crude oil. In the submerged cultivation in the glucose (3 g/L) containing medium, complete decolorization of indicator Resazurin was observed during two weeks in the presence of Bjerkandera adusta 139, C. unicolor 303, Coriolopsis gallica 142, P. ostreatus 70, P. pulmonarius 148, and T. versicolor 159. When artificially oil-polluted soil was inoculated with fungal mycelium pre-grown on a mixture of wheat straw and mandarin peels the maximum degradation rate (65%) was obtained when C. unicolor 305 was incubated in the 1% oil-containing soil for 28 days. At the same cultivation conditions, P. ostreatus 2175 eliminated 43.9% of initial oil when its concentration in the soil was increased to 2%. In the lignocellulose-containing soil, neither glucose nor yeast extract enhanced oil degradation, but wetting of soil with the distilled water to maintain its humidity favored oil elimination. The tested WRB secreted lignin-modifying enzymes in the presence of petroleum hydrocarbons; the higher was the concentration of lignocellulosic substrate in the soil the higher was the fungi enzyme activity. However, the data received did not show any direct relationship between the fungi enzyme activity and the degree of oil elimination.
Authors and Affiliations
Tina Jokharidze, Eva Kachlishvili, Vladimir Elisashvili
Keywords
Related Articles
- EP ID EP636005
- DOI 10.32006/eeep.2019.1.2636
- Views 99
- Downloads 0
LANDSCAPE PLANNING POSSIBILITIES FOR “KORIATA” SAND AND GRAVEL QUARRY, SOFIA REGION, BULGARIA
The paper presents results of landscape planning and investigation on Fluvisols. The object is “Koriata” sand and gravel quarry, Bulgaria. Soil samples were taken from seventeen soil profiles at a depth of 0-20 cm, 20-40...
BIODEGRADATION OF CRUDE OIL AND LIGNIN-MODIFYING ENZYME ACTIVITY OF WHITE ROT BASIDIOMYCETES
In the present study, the ability of eighteen white-rot basidiomycetes was evaluated for crude oil biodegradation. Cerrena unicolor strains, Panus tigrinus 433, P. lecometei 903, Pleurotus ostreatus 70, Trametes maxima 4...
FIRE SEVERITY ASSESSMENT USING NDVI DERIVED FROM LANDSAT TM/ETM IMAGES AND TERRAIN DATA
The forest fires influence on the plants and soil depends on the fire severity and time of exposure. Fire severity integrates physical, chemical and biological changes occurring in ecosystems on the area as a consequence...
EVALUATION OF COMPOSTING MATERIALS FOR SUSTAINABLE AGRICULTURAL APPLICATIONS
. Natural resources are elements of the environment that humans use as a means of production in order to achieve economic development. The aim of the study is based on the evaluation of the proposed 5 natural products /v...
APPLICATION OF REMOTE SENSING FOR HIGH MOUNTAIN ECOSYSTEM CONDITION ASSESSMENT (SOUTH WEST RILA MOUNTAIN - BULGARIA)
On basis of multispectral satellite data from Sentinel 2, an assessment of high mountain ecosystems condition is executed in selected territories of South West Rila Mountain. To define their actual condition, values of N...