BIOFILM FORMATION AND ANTIMICROBIAL RESISTANCE PATTERN AMONG UROPATHOGENS
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2015, Vol 4, Issue 2
Abstract
Background: Bacterial biofilms play an important role in urinary tract infections and is responsible for persistence infections and also the higher antimicrobial resistance is seen in biofilm forming uropathogen as compared to free floating bacteria. So the present study was undertaken with the aim to know the prevalence of biofilm formation and antimicrobial resistant pattern of biofilm producer and non-biofilm producing uropathogens. Materials & Methods: A total of 146 Gram negative bacilli and 62 S. aureus isolated from patients suspected UTIs were tested for biofilm formation and antimicrobial susceptibility testing by Kirby-Bauer disc diffusion method on Mueller Hinton agar as per CLSI guidelines. Result: Out of 208 isolates from urine, Biofilm formation was noted in 122(58.66%) and no biofilm formation in 86(41.35%).[Strong Biofilm formation in 76(36.54%) and weak biofilm formation in 46(22.12%)ï.In our study, we noted biofilm and non-biofilm forming microorganism showed mark difference in antimicrobial resistance pattern. In Staphylococcus aureus striking difference was noted to ciprofloxacin (100% versus 33.33%) and azithromycin (96%versus 41.67%). Isolates showed no resistance to linezolid. Whereas isolates of Pseudomonas aeruginosa to netilin (100% versus 42.86%).And in other Gram negative bacilli difference was noted to gentamicin (87.93% versus 13.43%) and norfloxacin (84.48% versus 37.31%) Conclusion: Biofilm forming isolates showed higher antimicrobial resistance as compared to non-biofilm producer. Thus, Uropathogen should be routinely screened for biofilm formation.
Authors and Affiliations
DardiCharanKaurG| Department of Microbiology, Maharashtra Institute of Medical Education & Research (MIMER), Talegaon Dabhade, Pune, Maharashtra, India, Corresponding author email: charan13@rediffmail.com, MaralSanjivani. S| Assistant Professor Symbiosis Institute of Health Sciences, Pune Maharashtra, India
SIGNET RING CELL ADENOCARCINOMA IN A URACHAL CYST-A RARE CASE
Adenocarcinoma arising in anurachal cyst is extremely rare. This paper describes a patient who came with chief complaints of hematuria, was treated with partial cystectomy for urachal cyst, and the pathologic examinatio...
Surgical therapeutic management of perforated peptic ulcer
Perforated peptic ulcer is an emergency should be readily corrected by surgical approach to reduce potential damage and the risk of mortality associated with the extension frame. The option of handling most commonly use...
Comparison of bloody pap smear fixation by Carnoy’s and fixator spray in samples from women with abnormal uterine bleeding
Abnormal uterine bleeding is a serious problem among women of late reproductive age. Cervical cancer is one of the reasons of AUB and Pap smear is the best way to diagnose it. Blood has negative effects on Pap smear es...
COMPARATIVE STUDY OF PULSE THERAPY WITH DAILY IMMUNOSUPPRESSIVE THERAPY IN STEROID RESPONSIVE DERMATOSIS
Background: Pemphigus, scleroderma and SLE are diseases of unknown etiology for which no specific treatment is effective. The introduction of corticosteroids and immunosuppressive drugs reduced the mortality rate. Obje...
A CASE REPORT OF MOLLUSCUM CONTAGIOSUM INFECTION IN AN HIV INFECTED INDIVIDUAL
Molluscum contagiosum is a viral disease caused by mollusci pox virus (DNA virus).Seen commonly as disease in children and immunocompromised adults. Infection transmitted by direct skin to skin contact, fomites or sexu...