Bioremediation of Polycyclic Aromatic Hydrocarbons by Bacillus Licheniformis ATHE9 and Bacillus Mojavensis ATHE13 as Newly Strains Isolated from Oil-Contaminated Soil
Journal Title: Journal of Geography, Environment and Earth Science International - Year 2017, Vol 11, Issue 2
Abstract
Removal of Polycyclic Aromatic Hydrocarbons (PAHs) from the environment is important because of their potentially deleterious affect human health. Biological methods that was applied in the present study is typically contain microbial processes. It is the most innocuous and effective solution that transform pollutant to nontoxic or less toxic substances. The objectives of the present study are enrichment, isolation and identification of PAHs degrading bacteria from oil-contaminated soil by molecular detection methods. Eight indigenous bacteria were isolated from oil-contaminated soils by using the enrichment methods. Two isolates with the highest optical density potentials were selected in media with 12.8 mg. L-1 of 16 PAHs as the sole source of carbon. They were identified as Bacillus licheniformis ATHE9 and Bacillus mojavensis ATHE13 by subjected to polymerase chain reaction with 16SrDNA primer and biochemically characterized. The bacterium strain including Bacillus licheniformis ATHE9 was isolated and removed by Acenaphtylene, Acenaphtene and Indeno pyrene in 72, 96 and 96 hours, respectively. The Bacillus mojavensis ATHE13 could remove Naphthalene in 72 hours and Acenaphtene, Acenaphtylene, Benzo(ghi)prylene, Dibenzo(ah)anthracene and Indeno pyrene in 96 hours. The results of this study strongly indicate that some of the indigenous bacteria have the potential role to remediate polycyclic aromatic hydrocarbons from oil-contaminated sites. These bacterial strains were able to grow in media with 12.8 mg. L-1 of 16 PAHs and reach the stationary phase. The results showed that using native bacteria with PAH-utilizing capabilities in contaminated sites could be useful for removal of these compounds from the environment.
Authors and Affiliations
Somayeh Eskandari, Mehran Hoodaji, Arezoo Tahmourespour, Atousa Abdollahi, Tahere Mohammadian Baghi, Saeid Eslamian, Kaveh Ostad-Ali-Askari
Keywords
Related Articles
- EP ID EP321417
- DOI 10.9734/JGEESI/2017/35447
- Views 64
- Downloads 0
2-D Electrical Resistivity Survey for Cassiterite Potential Mapping in Jos-Bukuru Area, North Central, Nigeria
Aim: To use 2-D Electrical Resistivity method in Cassiterite Potential mapping in Jos-Bukuru Area, North Central, Nigeria. Methodology: Locations suspected to have very high probability for cassiterite mineralisation fr...
The Emerging Population Increase and Its Environmental Challenges and Remedies in Iringa Municipal, Tanzania
The study has shown that the environmental challenges encountered in Iringa and most other urban areas in Africa are due to the anthropogenic activities arising from the influxes of migrants from rural areas. The urban a...
Geospatial Assessment of Uncontrolled Urbanization and Its Deviation from an Intelligent City: A Case Study of Fort Portal, Uganda
Uncontrolled urbanization is one of the major geo-environmental challenges facing the developing world today towards attainment of intelligent cities. Various studies have mainly concentrated on impact of uncontrolled ur...
Paleostress Analysis of Joints in Part of Basement Complex Area of Southwestern Nigeria
This study was carried out to understand structural features of joints of Owo area with the intention of unravelling the paleostress history of the area. The study area is a detachment of the Igarra schist belt, southwes...
Assessment of Hydrogeomorphic Suitability of Lake Mbemun for Socio-cultural Fishing Festival in the Bachama Chiefdom, Adamawa State, Northeastern Nigeria
The feasibility of socio cultural fishing in any fluviatile lake strongly depends on water and fluid mud depths of the lake. In this study, the suitability of Lake Mbemun for a socio cultural fishing festival has been ex...