Biosynthesis of Silver Nanoparticles (AgNPs) Using Bacteria Isolated From Heavy Metal Contaminated Soil Sample
Journal Title: GJRA-Global Journal For Research Analysis - Year 2015, Vol 4, Issue 5
Abstract
Recently the biological synthesis of nanoparticles and its therapeutic use has been increased. The uses of microorganisms such as bacteria, fungi and Actinomycetes as well as materials of animals and plant origin are reported to be used for the biosynthesis of nanoparticles. In present study an attempt has been made for the synthesis of silver nanoparticles using bacterial species isolated from heavy metal (Ag) contaminated soil. All the isolates were tested for Extracellular synthesis of Nanoparticle. The nanoparticles synthesized were screened adopting silver ion reduction test and was monitored by UV-Visible spectroscopy. The nanoparticles exhibited λ max in the range of 420-450nm, corresponding to the Plasmon resonance of silver nanoparticle. Amongst total 26 isolates, only 5 isolates viz., E.coli, Klebsiella, Enterobacter, Proteus, Pseudomonas, Species showed synthesis of Ag nanoparticle this indicates the potential of soil bacteria to reduce the silver salts. The present study indicates the possibilities of bacterial transformation method for the synthesis of Silver nanoparticle as an effective and economical method over chemical synthesis.
Authors and Affiliations
Payal Agrawal, Nikhilesh Kulkarni
Keywords
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