BIOSYNTHESIS OF SILVER NANOPARTICLES FROM FUNGAL ROOT ENDOPHYTES OF SIDA ACUTA PLANT AND EVALUATION OF THEIR ANTIBACTERIAL AND ANTIBIOTIC ENHANCING ACTIVITY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 11
Abstract
Objectives: Biosynthesis of silver nanoparticles has received much attention in recent years due to their numerous advantages over physical and chemical synthesis like being eco friendly, free from hazardous chemicals etc. Large number of biological agents are used for the biosynthesis of metal nanoparticles including plants and microbes. Amongst microbes fungi are better candidates for biosynthesis of various metal nanoparticles owing to certain physiological, morphological and metabolical characteristics. Further amongst fungi, the potential of endophytic fungi for the biosynthesis of metal nanoparticles has been less explored. Therefore, in present communication, the two important endophytic fungi isolated from Sida acuta (Wire weed) plant have been screened out for their potential to synthesize silver nanoparticles. The synthesized silver nanoparticles was further evaluated for antibacterial and antibiotic enhancing activity.Methods: The fungal root endophytes isolated from Sida acuta plants were identified by studying morphological and microscopical characterstics and were keyed out to their respective genus by consulting relevant literature. The silver nanoparticle synthesizing potential of the isolated fungal endophytes was observed visually and confirmed by UV/Visible spectroscopical analysis under the wavelength range of 380-680 nm. These silver nanoparticles were further characterized by transmission electron microscopy (TEM), particle size analyzer (PSA) and Fourier transform infrared spectroscopy (FTIR) analysis. In addition, their antibacterial and antibiotic enhancing activities against four standard bacterial pathogens (Escherichia coli, MRSA, Pseudomonas aeruginosa and Staphylococcus aureus) were also evaluated as per the standard disk diffusion method.Results: The two fungal root endophytes were isolated from Sida acuta plant and were identified as the species of Syncephalis and Geotrichum. Both these species were capable of synthesizing the silver nanoparticles as observed by the change in colour of the reaction mixture from transparent to dark brown and was confirmed by UV/Visible spectroscopy by observing the peaks of silver nanoparticles in the wavelength range of 380-680 nm. TEM analysis and study performed with particle size analyser revealed the silver nanoparticle synthesized by both the species varied in size and were mostly spherical in shape. While the FTIR analysis indicated the involvement of different functional groups in the silver nanoparticles synthesis by both the species. Besides, the synthesized silver nanoparticles was found to possess potential antimicrobial activity and was also capable of enhancing the efficiencies of some commercial antibiotics.Conclusion: The fungal root endophytes, Syncephalis and Geotrichum isolated from plant Sida acuta were capable of synthesizing silver nanoparticles with remarkable antibacterial and antibiotic enhancing properties.Â
Authors and Affiliations
Deepak Kumar Rahi, Aruna Singh Parmar, Vishal Tiwari
Keywords
Related Articles
- EP ID EP579280
- DOI -
- Views 69
- Downloads 0
PHYLOGENETIC STUDY OF THREE LINES CAVIA PORCELLUS (GUINEA PIGS) PERU, ANDINA AND INTI, "EL PRADO" FARM, UNIVERSITY OF THE ARMED FORCES – ESPE IN ECUADOR
Objective: The present study was undertaken to investigate the morphological and to establish molecular relations between three lines of guinea pigs, which are phenotypically selected and form the basis for a molecular m...
BETAMETHASONE DIPROPIONATE GEL FOR TREATMENT OF LOCALIZED PLAQUE PSORIASIS
Objective: The main aim of this study was to design and characterise betamethasone di-propionate loaded transfersomes (BD-T); as a topical formulation for the treatment of localized plaque psoriasis.Methods: A full facto...
EXTRACTION AND SUGAR COMPOSITION OF MUCILAGE IN URGINEA INDICA/DRIMIA INDICA (ROXB) KUNTH HYACINTHACEAE
Objective: Urginea indica (U. indica) Kunth. Hyacinthaceae has high mucilage content; the aim of the study is to isolate the mucilage and to analyze the sugar present in mucilage and its Physicochemical properties.Method...
SYNTHESIS AND ANTIVIRAL ACTIVITY OF NOVEL ETHYL 2-(3-HETEROCYCLE-1H-INDOL-1-YL) ACETATE DERIVATIVES
ABSTRACTObjective: Marek's disease (MD) is a widespread, herpesvirus-induced neoplastic disease in the domestic chicken that is caused by Marek's disease virus (MDV). Marek’s disease virus (MDV) belongs to the alphaher...
DEVELOPMENT AND VALIDATION OF ZERO AND FIRST ORDER SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF OPIPRAMOL IN BULK AND PHARMACEUTICAL DOSAGE FORM
Objective: Two simple, precise and accurate zero and first order spectrophotometric methods were developed and validated for the quantification of opipramol in bulk and tablet dosage form.Methods: The quantitative analys...