Biotic relations of Fusobacterium necrophorum in microbial associations in vitro
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2018, Vol 32, Issue
Abstract
Introduction. Scientific and practical studies on the study of the biotic properties of associations of microorganisms in vitro, namely, their qualitative and quantitative composition, the ratio of bacterial species and antagonistic, tolerant, symbiotic relationships between different species of these microorganisms are relevant. The goal of the work was to study in vitro the nature of the biotic relationships at the metabolic level between Fusobacterium necrophorum and isolates of microorganisms from microbial associations. Materials and methods. A modified method for in vitro determination the nature of the biotic relationships of Fusobacterium necrophorum with associations’ representatives in liquid media was used; pathogens were isolated from biomaterial of dead animals and their identification was carried out according to generally accepted methods. Results of research and discussion. Analysis of the results of conducted studies showed that all associated pathogens, isolated from biomaterial from dead animals, showed a symbiotic interactions regarding necrobacillosis causative agent, which was confirmed by stimulation of this pathogen growth in the presence of their metabolites. The highest level of necrobacillosis agent stimulation observed in the presence of metabolites of Staphylococcus aureus strain “Shakhtar”, since the accumulation of its bacterial mass significantly exceeded the similar control data by 2.2 times (p <0.001). A high level of symbiosis regarding F. necrophorum observed in Escherichia coli and Clostridium рerfringens types A, B and D, since the growth factor of the necrobacillosis pathogen in the presence of Escherichia coli metabolites was significantly higher by 25.0% (p <0.01), Clostridia by 10.8–14.3% (p <0.05), respectively, compared with the control indicators. Concerning other associate pathogens, the lowest level of F. necrophorum symbiosis observed with Clostridium рerfringens type C, Salmonella tyhyimurium and Salmonella enteritidis, which was confirmed by the quantitative accumulation of the bacterial mass of necrobacillosis pathogen during its cultivation in the presence of these cultures metabolites. Conclusion and prospects further research. Metabolites of all pathogens of Staphylococcus aureus, Escherichia coli, Clostridium perfringens types A, B, C, D, Salmonella tyrhimurium, Salmonella enteritidis, Salmonella Dublin, Salmonella typhisuis and Salmonella choleraesuis, isolated from dead animals biomaterial effected positively on the growth and reproduction of Fusobacterium necrophorum. Prospects for further studies will be focused on the study of the Fusobacterium necrophorum metabolites influence on the growth and reproduction of isolates of the association pathogens isolated from dead animals biomaterial, which will allow to obtain new scientific data on the conditions, nature and reasons of the infectious process development at necrobacteriosis of animals.
Authors and Affiliations
Alexander Zhovnir, Olga Gorbatiuk, Valentina Andriyaschuk, Galina Ryzhenko, Svetlana Tiutiun, Tatyana Ukhovska, S. Krylenko, L. G. Ulko
Keywords
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- EP ID EP389299
- DOI 10.31073/vet_biotech32(2)-17
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