Bmi1a Gene Expression changes in Gamma-Irradiated Zebrafish Embryos
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 1
Abstract
Bmi1 is an oncogene, which occurs in almost all human cancer and 50% of all tumors exhibit variations in their gene expression level. To further assessment of bmi1gene pathway involving in cancer, we have exploited the zebrafish which is a powerful vertebrate model system used to analyze the expression level during embryogenesis. Embryos were collected and treated with different doses of gamma radiation, for further observation. Zebrafish embryos were exposed to low dose rate of external gamma radiation from 5 to 25 Gy (with 5 interval dose rate) over a short period at 3hrs post fertilization with the objective of testing the appropriateness of 25 Gy guidelines as suggested by OECD. Total mRNA was isolated and cDNA was amplified with random primers by using Reverse Transcriptome PCR kit. Expression of two genes; BMI 1a & INK4A were quantified using specific Real Time PCR primers (q-PCR-Syber Green). qPCR showed decreased level at 10 Gy dose, increased expression level at 5 & 10 Gy irradiated embryos. Our present study examines different dose of radiation that enhances the, embryonic resistance to particular dose in which bmi1 expression level decreased when compared to control, 5 and 15 Gy. Lethality, mortality and morphological abnormalities were compared with bmi1 expression level.Hence the present attempt to identify those radiosensitive pathways in the zebrafish would certainly help to choose the target genes.
Authors and Affiliations
Anandhi Manickam¹, Ranjithkumar Ganeshan¹, Natarajan Thillainathan, Ramkumar Rajendiran, Perumal Pachiappan*
Keywords
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