C-peptide Attenuates Progression of Atherosclerosis in Late Stages of Type 2 Diabetes in Male Albino Rats
Journal Title: Cardiology and Angiology: An International Journal - Year 2016, Vol 5, Issue 4
Abstract
Aims: Study the role of C-peptide in development of atherosclerosis in late stages of type 2 diabetes in rats. Methodology: Late stages of type II diabetes were induced by administeration of high fat diet and intraperitoneal injection of a single dose of streptozotocin 35 mg/kg, then rats were divided into 5 groups: 1) control, 2) Diabetic (DM), 3) Diabetic + C-peptide, 4) Diabetic + L-NAME, and 5) Diabetic + C-peptide + L-NAME. Measurements: At the end of the experiment blood samples were taken for measurement of serum glucose and insulin levels. The arch of the aorta was taken for: Histopathological study and measurement of tissue lipid peroxides (MDA), Bcl-2 (B-cell lymphoma protein 2, apoptotic factor), nitric oxide (NO) and tumor necrosis factor-α (TNF-α). Results: Aorta of diabetic rats showed severe atherosclerotic changes, and there were significant increase in serum glucose, tissue MDA, and TNF-α alongside there were significant decrease in serum insulin, tissue NO and Bcl-2 as compared to control group. Significant improvement in atherosclerotic changes were observed in Diabetic + C-peptide group which was accompanied with significant decrease in serum glucose, tissue MDA, and TNF-α. There were also significant increase in serum insulin and tissue NO and Bcl-2 as compared to DM group. In DM + L-NAME group, there were severe deterioration of atherosclerotic changes, and there were significant increase in serum glucose, tissue MDA, and TNF-α alongside significant decrease in serum insulin, tissue NO and Bcl-2 as compared to DM group. Although adding L-NAME to DM + C-peptide group abolished increasing in serum insulin level but improvement in atherosclerotic changes and other parameters were still present. Conclusion: C-peptide has a protective effect against development of atherosclerosis through many mechanisms including hypoglycemic, antioxidant, antiapoptotic, and anti-inflammatory effects. So administration of C-peptide as an adjuvant therapy in late stages of type II diabetes can significantly decrease incidence of vasculopathy.
Authors and Affiliations
Alaa El Din R. Abdel Rheim, Hanaa M. Ibrahim, Azza Hussein Ali
Keywords
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- EP ID EP327686
- DOI 10.9734/CA/2016/30230
- Views 85
- Downloads 0
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