Cadmium Induced Toxicity in Human Peripheral Blood Lymphocytes Culture and Its Amelioration by Vitamin C
Journal Title: Annual Research & Review in Biology - Year 2017, Vol 20, Issue 1
Abstract
Introduction: Cadmium is a ubiquitous and toxic environmental pollutant increasing worldwide due to high consumption in various industries. At the cellular level, cadmium affects cell proliferation, differentiation, apoptosis and other cellular activities. In contrast to cadmium, vitamin C (ascorbic acid) is a powerful reducing agent known to capable of rapidly scavenging several reactive oxygen species (ROS). Aim: Hence, the present work aimed to investigate the cadmium chloride (Low dose-10-6 M; Mid dose-10-5 M; High dose-10-4 M) induced cytotoxic and genotoxic effects in human peripheral blood lymphocyte culture (PBLC) of healthy individuals (n=10) and its amelioration by vitamin C (10-5 M). The study was divided in various groups consisting of control, vitamin C, cadmium alone, cadmium with vitamin C and ethyl methanesulfonate as positive control. Results: After genotoxicity indices and free radical toxicity parameters evaluated, results showed alteration in free radical toxicity indices and genotoxic indices in cadmium exposed cultures. Although vitamin C co-exposure revealed protective effects against genotoxicity by scavenging free radicals and balancing the activity of antioxidant defense system with decreasing in level of lipid peroxidation. Conclusion: Vitamin C led to significant decrease in cadmium induced toxicity and substantial amelioration was observed in all studied parameters.
Authors and Affiliations
Ambar Pathan, Ankit Nariya, Naumita Shah, Alpesh Patel, Shiva Chettiar, Devendrasinh Jhala
Keywords
Related Articles
- EP ID EP314923
- DOI 10.9734/ARRB/2017/37837
- Views 86
- Downloads 0
Interaction between Phytoplankton and Zooplankton in Some Freshwater Bodies in Relation to Some Ecological Factors in Taif, KSA
Two water bodies in Taif city, Wadi Al-Arj and Saiysad, were investigated between December and February (wet season) and June to August, 2016 (Dry season) to investigate the community structure of fresh water phytoplankt...
Dissolving Ability of Commercialized White Tea on Chocolate-stained Fabric: A Potential Stain Remover
Tea, being the second most consumed beverage in the world, produces huge amount of waste when the used tea bags are readily discarded. This study determined the change in the relative absorbance of commercialized white t...
In vitro Regeneration of Banana and Assessment of Genetic Fidelity in the Regenerated Plantlets through RAPD
Amrit Sagar belonging to banana (Musa acuminata) genome group AAA is popularly grown in North Eastern part of India for its high yield and natural disease resistance potential. The explants were established initially on...
Robert’s Syndrome in an 8 Month Infant
Background: Robert’s syndrome is an extremely rare autosomal recessive genetic disorder. The Gene of this syndrome is located on chromosome 8 and it is characterized by craniofacial anomalies and deformity in the limbs....
Bacteriocins Contributing in Rhizospheric Competition among Fluorescent Pseudomonads
Aims: To examine the production of bacteriocins through the study of a group of rhizospheric Pseudomonas isolates already known to produce metabolites that are antagonistic to fungi. Methodology: Fourteen rhizospheric st...