Callus formation and somatic embryos of Cocos nucifera L. cv. Yellow Indian in semi-solid culture medium
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 3
Abstract
The objective of this work was evaluated the effect of the concentration of 2,4-D and the type of explant in the formation of callus of Cocos nucifera L. cv. Indio Amarillo. The biggest percentages of explants with callus were obtained with the sections 1 in all the 2,4-D concentration, with a gradual decrease when the section belongs to the most external region of the somatic embryo caulinar zone. The best results in the callus formation process was obtained with the 2,4-D concentrations of 28.0 mg.l-1 and 35.0 mg.l-1, with 90% and 70% of the explants with callus and an average level of development according to the scale of 3.5 and 2.2 at ten weeks of culture. With the objective of to form somatic embryos, the callus obtained with 28.0 mg.l-1 of 2,4-D were put in a culture medium of similar composition to the callus formation medium, but with a lower concentration of 2,4-D (21.0 mg.l-1) and at five weeks of culture was observed as average seven somatic embryos per callus, some of them germinated.
Authors and Affiliations
Igor Bidot, Manuel de Feria, Alina Capote, Maité Chávez, Elisa Quiala, Raúl Barbón, Naivy Pérez-Alonso, Juan C. Pérez
Keywords
Related Articles
- EP ID EP697805
- DOI -
- Views 45
- Downloads 0
New explant for somatic embryogenesis induction and plant regeneration from diploid banana (‘Calcutta 4’, Musa AA)
A m et hod has b een devel oped f or plant regeneration vi a em bry ogenic c ell suspensions from dipl oi d cult ivar ‘Cal cutt a 4 ́. For callus induction with embryogenic structures, different plant tissues such as sc...
Induction and callus formation of Anthurium andraeanum variety ´Lambada ’ from foliar sections of in vitro plants
Foliar sections of in vitro plants of Anthurium andraeanum variety ‘Lambada’ were used as explants for the induction and formation of callus. The formation of callus was evaluated in two culture mediums (MS and Nitsch,...
Optimal concentration of Ethyl Methane Sulfonate for phenotypic variation induction in Phaseolus vulgaris L. cv. ‘DOR 364’
Common bean (Phaseolus vulgaris L.) is the main legume used for human consumption worldwide and is an important objective in breeding programs to favor certain characteristics of agronomic interest. In order to determi...
Identification and control of Bacillus sp., Contaminant of in vitro establishment of Guadua angustifolia Kunth
Efficient protocols of Guadua angustifolia Kunth micropropagation are not reported due to two principal problems for establishing it: explants necrosis and microbial contamination. In this work a high frequent bacterial...
Effect of adding different concentrations of activated carbon on the in vitro multiplication of yams
Phenolic oxidation can cause serious problems in the establishment, multiplication and survival of explants on most of plant species. The present investigation was developed toevaluate the effect of adding different conc...