Candida albicans isolated from urine: Phenotypic and molecular identification, virulence factors and antifungal susceptibility
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 7
Abstract
Objective: To isolate Candida albicans (C. albicans) from the urine of hospitalized patients and assess the virulence factors and antifungal susceptibility profiles of the isolates. Methods: Yeasts were identified using the chromogenic medium CHROMagar™, the VITEK® 2 system, hypertonic Sabouraud broth, tobacco agar, polymerase chain reaction, and DNA sequencing. The evaluated virulence factors were proteinase production, phospholipase production, and biofilm production on polystyrene. The broth microdilution technique was used to determine the minimum inhibitory concentration. Results: All yeasts isolated from urine were identified as C. albicans using both classical and molecular methods. Although 91.3% of the isolates showed no phospholipase activity, 56.5% showed strong proteinase activity and 91.7% produced biofilm. All microorganisms were sensitive to fluconazole, voriconazole and amphotericin B, but 56.5% of the yeasts showed resistance to itraconazole. Conclusions: C. albicans isolates from urine have a high capacity for virulence and can be associated with infectious processes. Furthermore, the high percentage of isolates resistant to itraconazole is important because this antifungal agent is commonly used to treat fungal infections in the hospital environment.
Authors and Affiliations
Laura Wiebusch, Bruna de Paula Bicudo, Adriana Araújo de Almeida-Apolonio, Luana Mireli Carbonera Rodrigues, Danielly Beraldo dos Santos Silva, Danielle Ferreira Lonchiati, Renata Pires de Araujo, Alex´eia Barufatti Grisolia, Kelly Mari Pires de Oliveira
Keywords
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- EP ID EP269192
- DOI 10.1016/j.apjtb.2017.06.006
- Views 75
- Downloads 0
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