Cardiac Tolerance of An Ethyl Acetate Extract of Holarrhenafloribunda (G. Don) Durand and Schinz Leaves in Wistar Rats
Journal Title: Scholars Academic Journal of Pharmacy - Year 2017, Vol 6, Issue 6
Abstract
Holarrhena floribunda is a plant used in traditional medicine for the treatment of various pathologies. However, it could cause toxic effects on the heart of patients. In order to determine its possible cardiotoxic effects, forty rats were randomly grouped into 2 (control group and treated group), combined with 10 rats (control satellite group and satellite treated group). Treated and satellite treated groups received, by gavage, 1000 mg / kg b.w. of the ethyl acetate extract of the leaves of H. floribunda(EAHf). Control and satellite control groups were administered orally with distilled water for 90 days. Animals blood samples were taken a day before the beginning of administration of H. floribunda and every week during the first month and at the end of every month from the second month until the end of the experiment. Serum was prepared for cardiac biochemical markers assay. The rats of the treated group at the end of the third month and those of the satellite group at the end of the fourth month were euthanized using overdose of ether, the heart of the rats was removed for gross morphology and histopathology analysis. The results of the biochemical parameters showed that this extract induced a significant decrease (p <0.05) in total cholesterol, calcium, potassium and a significant increase (p < 0.05) of HDL, sodium levels, and AST activities. On the other hand, ALT, LDH and CPK activities, and serum triglyceride concentrations did not show any significant changes (p <0.05) in treated rats group compared to controls. Microscopic observations of heart tissue sections of rats treated with the ethyl acetate extract of H. floribundashowed no lesions, no edema, and no necrosis. These results suggest that H. floribunda did not interfere with functioning or altered the integrity of the heart. Keywords:Cardiac bio-tolerance, Holarrhena floribunda, histopathology, wistar rats.
Authors and Affiliations
D. D. Koudou, Oussou J. B, Tra-Bi Irie Otis, Kone Mama, A. P. Yapo, A. J. Djaman
Keywords
Related Articles
- EP ID EP378140
- DOI -
- Views 82
- Downloads 0
The Effect of Feeding Commercial Oil of Black Cumin in Sheep
This study investigated the effects of feeding commercial oil of the Black cumin (seeds of Nigella sativa) on the levels of total cholesterol and the body weights in sheep. Twelve male cross breed sheep were divided indi...
Effect of Long Chain Polyunsaturated Fatty Acid (LC-PUFA) Transfer across Human Placenta on Maternal and Fetal Outcome
Long chain polyunsaturated fatty acids (LC-PUFAs) such as docosahexaenoic acid and arachidonic acid have important role in maternal and fetal development. Several studies have provided link between docosahexaenoic acid (...
Analytical method validation and quantification of raloxifene hydrochloride and its related substance in API using reverse phase - liquid chromatographic method
The present paper describes the reverse phase-high performance liquid chromatographic method and was validated as per ICH guidelines for the determination of related substances in raloxifene hydrochloride. RP-Liquid chro...
Development and Evaluation of AVSER Matrix Analysis of Inventory Control Technique for Community Pharmacy Practice in a Tertiary Care Hospital
Approximately 40% of annual hospital budget is spent on drug procurement. The objective of the study was to design and evaluate suitable inventory management formula for a community pharmacy attached to a tertiary care g...
From Empirical to Scientific: Microbiological Activity of Black Tea Leaves (Camellia sinensis L.) Infusion from Subang Indonesia as Antidiarrhea
Abstract:Subang area is one of the largest tea producers in Indonesia with black tea as a product favored of the Indonesian people. Besides being enjoyed as a drink, empirically black tea leaves infusions were used as an...