Cellular localization of orexin receptors in human pituitary.
Journal Title: Journal of Clinical Endocrinology and Metabolism - Year 2001, Vol 86, Issue 7
Abstract
Orexins-A and -B are hypothalamic peptides derived from a precursor calledprepro-orexin and relationated with the stimulation of food intake. They act on G protein receptors namedorexin receptor 1 (OX(1)R) and orexin receptor 2 (OX(2)R), respectively. In the present study, we usedRT-PCR and immunohistochemical techniques to detect the presence of OX(1)R and OX(2)R in human pituitary.A band of the expected size for both OX(1)R and OX(2)R was shown in human pituitary by RT-PCR. The cellularlocalization of OX(1)R and OX(2)R was carried out using histological techniques. By consecutive sectionswe demonstrated that OX(1)R was present in acidophil, diffusely distributed cells, which represent thehalf of the total adenohypophysis cell population. As was expected, these cells were shown to coexpressGH. OX(2)R was found in the pars intermedia and in clusters of basophil cells of the anterior pituitary,which coexpress ACTH. These results were confirmed by double immunofluorescence techniques. We also foundfocal positivity in axon terminals of neurohypophysis, more intense for OX(2)R than for OX(1)R. In conclusion,these results demonstrated for the first time that OX(1)R and OX(2)R were expressed by somatotrope andcorticotrope cells, respectively.
Authors and Affiliations
R SeñarIs, T GarcIa-Caballero, G Morel, M Blanco, F Casanueva, C Diéguez, A Beiras
Keywords
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