Changes in Fatty Acid Composition in Leaf Lipids of Canola Biotech Plants under Short-time Heat Stress
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2014, Vol 10, Issue 2
Abstract
In order to study the influence of expression of heterologous genes of different origin (cyp11A1 and desC) on canola thermotolerance improvement on leaf membrane level the fatty acid composition was analyzed under short-time heat test. Cyp11A1 gene encodes cytochrome P450SCC from bovine adrenal cortex mitochondria and was shown to affect the biosynthesis of steroid compounds. DesC gene encodes ∆9-acyl-lipid desaturase of cyanobacterium Synechococcus vulcanus. Decrease in palmitlinolenic acid content and index unsaturation as well as increase in total fatty acid and palmitic acid content were identified in cyp11A1 canola in comparison with wild-type plants in stressfull conditions. But control and desC plants demonstrated similar changes in saturated (16:0), trienoic (16:3 and 18:3) fatty acid quantity, total fatty acid content and index unsaturation. Heterologous desC gene expression did not influence fatty acid composition and did not give advantages for plant under heat. Integration of cyp11A1 gene in canola led to thermotolerance improvement on membrane level.
Authors and Affiliations
Liudmyla Sakhno, Mariia Slyvets, Nataliia Korol, Andriy Ostapchuk, Yuriy Sheludko, Mycola Kuchuk
Keywords
Related Articles
- EP ID EP110414
- DOI -
- Views 106
- Downloads 0
Salinity Induced Changes in β-carotene, Thiamine, Riboflavin and Ascorbic Acid Content in Spinacia oleracea L. var. All Green
Vitamins are the functional components of various enzyme-regulated biochemical reactions occurring to create energy. Vitamin contents of plants are known to show altered metabolism under the influence of salinity. Not mu...
ДИАГНОСТИКА ВОЗБУДИТЕЛЯ КОЛЬЦЕВОЙ ГНИЛИ В КЛУБНЯХ КАРТОФЕЛЯ МЕТОДОМ ПОЛИМЕРАЗНОЙ ЦЕПНОЙ РЕАКЦИИ
Бактериальная кольцевая гниль, вызываемая [i]Clavibacter michiganensis ssp. sepedonicus[/i], - широко распространенное заболевание картофеля, наносящее значительный экономический ущерб сельскому хозяйству и семеноводству...
Impact of Cold Stress on Physiological, Hormonal and Immune Status in Male and Female Broad Breasted White Turkeys
In this study physiological, certain blood hormones and immune status of male and female Broad Breasted White turkeys were recorded under cold stress. Birds were acclimated two weeks prior to the start of experiment and...
Small HSPs molecular weights as new indication to the hypothesis of segregated status of thermophilic relict Gmelinoides fasciatus among baikal and palearctic amphipods
Among the great diverse of Baikal fauna of amphipods (more than 350 species and subspecies) Gmelinoides fasciatus (Stebbing 1899) takes the special place. It is characterized by a high morphological variability and wide...
Salicylic Acid Ameliorates the Effects of Oxidative Stress Induced by Water Deficit in Hydroponic Culture of Nigella sativa
Osmotic stress associated with drought, and salinity is a serious problem that inhibits the growth of plants, mainly due to disturbance of the balance between production of ROS and antioxidant defense and causing oxidati...