Chaperone DnaJ Influences the Formation of Biofilm by Escherichia coli
Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 3
Abstract
DnaJ chaperone, a member of the so called DnaK-DnaJ-GrpE chaperone machine plays an important role in cell physiology. The ability of Escherichia coli ΔdnaJ mutant to form biofilm was studied. It was shown that this mutant is impaired in biofilm development when exposed to 42°C for 2 h. The impairment in biofilm development was observed when the heat shock was applied either at the onset of biofilm formation or 2 h later. The biofilm formed was thinner and its structure was changed as compared to wild-type strain. This defect could be complemented by the introduction of a wild-type gene on a low-copy plasmid.
Authors and Affiliations
Anna Grudniak, Jolanta Włodkowska, Krystyna Wolska
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