CHARACTERISATION OF A BACTERIUM LIPASE (Bacillus subtilis) FROM VEGETABLE OIL POLLUTED SOIL
Journal Title: International Journal of Applied and Natural Sciences - Year 2018, Vol 7, Issue 5
Abstract
Bacteria are very useful in protecting the environment. The lipolytic activities of physiologically diverse bacteria have great potential to degrade oil spills in the environment. There is need for extensive characterisation of the bacterium lipase for the treatment of vegetable oil-polluted sites. This work was carried out to preliminarily characterise the lipase of Pseudomonas fluoresens. Pseudomonas fluoresens was screened for lipase production using standard methods. Temperature, pH, ion concentration (NaNO3 and MgSO4), enzyme concentration, nitrogen concentration, substrate concentration, time course and agitation speed were optimised for the lipase activity as well as growth. Crude enzyme of Pseudomonas fluoresens had the highest lipase activity and growth of 0.8 U/mL and 1.418 mg/mL respectively at room temperature, but when production was optimised higher activity 0.9 U/mL was seen in the use of glucose as substrate. Agitation with the speed used did not support lipase production but supported growth (1.998 mg/mL) at agitation speed of 100 rpm. Different substrate concentration which had optimum enzyme activity for Pseudomonas fluoresens had activity of (1.1 U/mL), was at 1.0 %. At 30oC Pseudomonas fluoresens had activity of 1.0 u/mL. Substrate concentration had optimum growth (1.778 mg/mL) at 0.5 %. The enzyme has good potential for the hydrolysis of vegetable oils, which is an important factor in environmental cleanup of vegetable oil spill site.
Authors and Affiliations
Popoola B. M
Keywords
Related Articles
- EP ID EP371382
- DOI -
- Views 107
- Downloads 0
To Study Various Health Care and Safety Facilities Provided at Construction Sites to Cement Porters
Cement porters often work in dusty circumstances with high labour intend sites. Cement can cause ill health mainly by skin contact, inhalation of dust and manual handling. Exposure to cement dust may cause irritation of...
Detection New Strains and Molecular Study of the Oprl Gene of Pseudomonas Aeruginosa Isolated from Clinical Sources in Thi-Qar Province
The study was conducted for a period between March and September 2016.A total of 314 samples various clinical cases of different patients were randomly collected and examined for detection of Pseudomonas aeruginosa. Thes...
Phytochemical Screening of in Vitro and in Vivo Grown Plantlets of Bacopa Monnieri L
Different types of extracts of in vivo and in vitro grown plantlets of Bacopa monnieri L. were qualitatively evaluated to identify the chemical constituents like saponins, alkaloids, resins, Quinones, tannins, phenols, f...
PETROGRAPHY, IDENTIFICATION OF METASOMATIC TEXTURES AND ISOCHEMICAL REACTIONS FROM DARBA SUITE, SW JORDAN
Hydrothermal fluids penetrated Darba rocks through their weak points in the solid state during the latest stage of their crystallization. Such fluid movement was facilitated by tectonic deformation. The Ediacaran rocks o...
ASSESSMENT OF BEEKEEPING PROBLEMS IN JADA LOCAL GOVERNMENT AREA OF ADAMAWA STATE, NIGERIA
The study examined Honey Production Problems in Jada Local Government Area of Adamawa State, Nigeria. Three council Wards were randomly selected for the study. Structured questionnaires were administered among the respon...