Characterisаtion of exo-inulinase concentrates from newly isolated thermophilic Bacillus strains - Bacillus sp. SG113 and Bacillus sp. SG115
Journal Title: Journal of BioScience and Biotechnology - Year 2017, Vol 6, Issue 3
Abstract
Enzyme concentrates with inulinase and invertase activity were derived from strains Bacillus sp. SG113 and Bacillus sp. SG115. The enzyme concentrate from strain Bacillus sp. SG113 has two distinct temperature optima – 50°С and 65°С for inulinase activity and 55°С and 65°С for invertase activity. Both activities are 100% sustainable for an hour at 60°С and 65°С. The inulinase activity of the enzyme concentrate from strain Bacillus sp. SG115 has two pH optima – pH 5.0 and pH 9.0, whilst invertase activity has only one – pH 6.0. The enzyme is stable at pH 7.0 (inulinase activity) and pH 6.0 (invertase activity). The concentrate from strain Bacillus sp. SG113 hydrolyses inulin completely for 60 minutes at 60°С and 65°С. The invertase in the concentrate hydrolyses saccharose completely at 65°С, and partially (50%) at 70°С. Inulin extracts from topinambour, onion and garlic are almost entirely hydrolysed. The enzyme from strain Bacillus sp. SG115 hydrolyses to a lesser extent the studied inulin extracts and it was observed that hydrolysis was effective for 40 minutes. Thereafter the amount of reducing sugars does not increase, presumably due to product inhibition. TLC data suggests that enzymes from strain Bacillus sp. SG113 and strain Bacillus sp. SG115 completely degrade inulin, onion and garlic extracts, whilst only partially degrading raffinose, with fructose being the end product in all cases, which proves their exo-activity. However, the above-mentioned enzymes do not hydrolyse melezitose.
Authors and Affiliations
Viara Ivanova, Simeon Gavrailov, Veronica Pashkoulova
Keywords
Related Articles
- EP ID EP603244
- DOI -
- Views 81
- Downloads 0
Aberrant signature patterns of ATM, γ-H2AX and p53 proteins in the patients exposed to methyl isocyanate diagnosed with gallbladder cancer
Cancer of gallbladder is a hidden phenomenon and highly malignant with underprivileged diagnosis and poor survival. Study of cancer patterns amongst victims of Bhopal gas tragedy exposed to methyl isocyanate reveal...
Evaluation of radiation use efficiency of common bean ([i]Phaseolus vulgaris[/i] L.) cultivars as affected by plant density under Mashhad climatic conditions
In order to determine the effect of different plant densities in bean cultivars, an experiment was conducted as factorial layout based on a randomized complete block design with three replications at Agricultural Researc...
Validation of internal control genes for quantitative real-time PCR under different experiment conditions in Multiple Myeloma
Multiple myeloma (MM) is a cancer of malignant plasma cells in the bone marrow which might lead to the development of one or more clinical manifestations such as bone destruction, anemia, renal insufficiency etc. Gene ex...
Genomic changes in speciation of the family Chironomidae, Diptera
The mode of speciation adopted by a species or group of related species of animals (insects) is clearly determined to a certain extent by the architecture of their genetic system. Chromosome rearrangements, the loc...
Influence of composition on the biological activity of pectic polysaccharides from leek
Water-soluble and acid-soluble pectic polysaccharides with significant immunostimulating activity were isolated from alcohol-insoluble solids (AIS) of leek. Pectic polysaccharides characterized with high anhydrouronic ac...