CHARACTERISTIC FEATURES OF КЕY-LIKE BACTERIOPHAGES OF ERWINIA AMYLOVORA
Journal Title: Мікробіологія і біотехнологія - Year 2017, Vol 0, Issue 4
Abstract
Aim. To study the heterogeneity of phage isolates obtained from twigs and leaves of pear affected with Erwinia amylovora. Methods. The heterogeneity of phage isolates was studied using the system included low pressure liquid chromatography (LPLC), electron-microscopy analysis of virions and DNA restriction analysis. In this work, the bacteriophages KEY, KEY/7 and KEY/25 were used. Results. the isolate contains a two-component population of KEY-like phage particles, which differ in affinity for DEAE-cellulose. Phages of both subpopulations are represented by virions of B1 morphotype (Siphoviridae family) with regular icosahedral capsids with the diameter of about 77 nm and the length of the caudal appendages close to 172 nm. These phage particles are similar to those of the prototype KEY phage in morphology and size. According to the restriction analysis data, the genomes of the pure line phages KEY/7 and KEY/25 are similar to each other and have the size of about 72 kb, which is 25–27% less than the size of the genome of KEY phage. It is assumed that the phages KEY/7 and KEY/25 represent deletion variants of KEY phage. Conclusions. The heterogeneity of phage isolates get from damaged plants have two main characteristics:the changes of affinity of particles to DEAE-cellulose and the changes of genetic structure of its genome. Further research both phage isolates and phage population are important for study of ecology and opportunity of phage using for biocontrol of dangerous woody plants pathogens.
Authors and Affiliations
А. А. Boyko, G. І. Zhuminska, А. І. Kushkina, V. О. Ivanytsia, F. I. Tovkach
Keywords
Related Articles
- EP ID EP316222
- DOI 10.18524/2307-4663.2017.4(40).118258
- Views 124
- Downloads 0
VIETNAMESE FERMENTED PLANTS LACTOBACTERIA ANTAGONISTIC ACTIVITY
The aim of the research was to identify and study antagonistic properties of lactic acid bacteria isolated from vietnamese fermented plant products. Materials and methods. Lactobacillus genus bacteria strains were isolat...
BIOTECHNOLOGICAL METHODS FOR PROCESSING SUBSTANDARD ORES AND WASTES
The information about using of biotechnological methods for the processing of mineral and technogenic raw materials was analyzed and summarized in the article. A review of the main groups of microorganisms that make up t...
VIRION DNAs STRUCTURE OF TEMPERATE ERWINIOPHAGES 49 AND 59
The aim was to conduct a comparative study of the phages 49 and 59 virion DNAs. Methods. Properties of phage DNA were studied using the standard restriction and bioinformatic analyses. Results. The genomes of erwiniaphag...
ANTAGONISTIC ACTIVITY OF MACROMYCETES AGAINST MUCOR SP IFBG 139
Aim. To screen macromycetes for their ability to show antagonistic activity against Mucor sp. IFBG 139 micromycete. Methods. Antagonistic activity of the representatives of 30 macromycetes against Mucor sp. IFBG 139 has...
ANTAGONISTIC ACTIVITY OF SOIL STREPTOMYCETES AGAINST PHYTOPATHOGENIC BACTERIAAND FUNGI
Aim. The selection of soil active cultures of Streptomyces antagonists promising for the development of a biopreparation for plant protection against pathogens of fungal and bacterial diseases. Methods. Streptomycetes we...