Characterization and Expression Analysis of WRKY Transcription Factors in Groundnut (Arachis hypogaea L.)
Journal Title: International Journal of Biochemistry Research & Review - Year 2017, Vol 17, Issue 1
Abstract
Groundnut (Arachis hypogaea L.), is an important subsistence oil yielding crop of the semi-arid tropics and often exposed to several environmental cues (high temperature, drought & heavy metal). The WRKY transcription factor (TF) is one of the master regulator, and play vital role in stress responses. However, far less information is available on functional characterization and tolerance mechanism of stress responsive WRKY genes in groundnut till date. In this study, a comprehensive phylogenetic, protein features, gene structure and motif analysis of WRKY TF gene family was carried out. We conducted expression profiling of 10 WRKY genes under high temperature, drought and heavy metal (CdCl2) in various tissues. Majority of the AhWRKY (Arachis hypogaea WRKY) proteins were clustered and share close relationship with Arabidopsis and Glycine max. RT- qPCR analysis of AhWRKY genes revealed differential expression either in their transcript abundance or in their expression patterns in response to at least one abiotic stress. In particular, AhWRKY41 expression level was found to be maximum in all the stress conditions. On the other hand, AhWRKY20 and AhWRKY22 were down regulated. The obtained data demonstrate that AhWRKY41 may act as a positive regulator in drought/ high temperature/ heavy metal and would exhibit stress tolerance mechanism by activation of stress-associated gene expression.
Authors and Affiliations
B. Suchithra, V. R. Devaraj, Jyotirmoy Ghosh, R. Nageshbabu
Keywords
Related Articles
- EP ID EP316983
- DOI 10.9734/IJBCRR/2017/32784
- Views 119
- Downloads 0
In-vivo Antiplasmodial Activity of Aqueous, N-Butanol and Ethylacetate Fractions of Leaf and Stem Bark Methanol Extracts of Diospyros mespiliformis on Plasmodium berghei berghei (Nk65) Infected Mice
Aim: To determine the in-vivo antiplasmodial activity of aqueous, N-butanol and ethylacetate fractions of leaf and stem bark methanol extracts of Diospyros mespiliformis on Plasmodium berghei berghei (Nk65) infected mice...
Effect of Storage Temperature on the Nutritional Compositions of Lactic Acid Bacteria Fermented Spicy Tigernut- Milk Drink
The fundamental benefit for consuming edible products is based on its nutritional compositions. Several factors affect the nutritional composition of diverse edible products. This study was carried out between August-Oct...
Estimation of the Modulatory Roles of Thieno [2,3-c] Pyrazole Compounds Versus the Toxicity of 4-Nonylphenol in African Catfish (Clarias gariepinus)
The endocrine disrupting substances represent major challenge to aquaculture and the most widespread one is 4-nonylphenol (4-NP). Pyrazole possess an interesting broad spectrum of pharmacological actions. Therefore, this...
Study of Thyroid Dysfunction in Association with Infertility
Aims: To evaluate the relation of female infertility to thyroid dysfunction. Study Design: This is a descriptive, hospital-based study. Place and Duration of Study: The present study was carried out in the department of...
The "Syndrome of Cardiogenic Insulin Resistance"
Hyperglycaemia in patients admitted in intensive care units (ICU) with acute myocardial infarction is a common phenomena observed. This unique situation of cardiogenic insulin resistance does not spare diabetics or non -...