Characterization and Invitro Cytotoxicity of T-2 Toxin Isolated from Corn
Journal Title: International Journal of Current Research and Review - Year 2018, Vol 10, Issue 1
Abstract
Aim: Main objective of the study is to characterize and evaluate invitro cytotoxicity of T-2 toxin isolated from corn. Methodology: The fungal strain was isolated by standard blotter method; morphological identification was done using scanning electron microscope (SEM). The fungal strain was identified as F. sporotrichioides based on its cultural and morphological characteristics and internal transcribed spacer (ITS/ITS4) primers. The toxin was characterized using thin layer chromatography (TLC), HPLC and Fourier Transform Infrared Spectroscopy (FTIR) Result: 100ml of culture filtrate yielded 10.126µg of toxin. The functional groups of toxin were identified by (FTIR) method. Invitro cytotoxicity of T-2 toxin was evaluated on human keratinocytes, hepatoma and neuron cell lines. HaCaT and HepG2 cells were found to be more sensitive than SHSY5Y to T-2 toxin exposure at 50-100ng and 100-200ng. Discussion and conclusion: T-2 toxin was extracted and characterized from corn samples, by analytical and molecular methods in addition FTIR analysis verifies the functional groups of T-2 toxin. Further invitro cytotoxicity was evaluated using different human cell lines. Comparably skin and liver cell lines found more sensitive to T-2 toxin exposure. Sensitivity of the cell lines to the toxin at ng may be due to the purity of the toxin
Authors and Affiliations
Dr. Farhath Khanum
Keywords
Related Articles
- EP ID EP533253
- DOI 10.7324/IJCRR.2018.1019
- Views 126
- Downloads 0
Virtual Screening and Docking Analysis of Novel Flavonoidanalogues as Antipsoriaticagents
Introduction: Psoriasis is an immune-mediated chronic, inflammatory skin disease characterized by hyper proliferative keratinocytes and their infiltration into the dermis of T cells, dendritic cells, macrophages and neut...
A Case Report of Rare Fungal Lesion
A Case Report of Rare Fungal Lesion
TWO NEW DEMATIACEOUS FUNGI FROM WEST BENGAL, INDIA
This communication deals with the description and illustrations of two undescribed species of Cercosporoid fungi viz.Cercospora krishnathii Haldar sp.nov. and Cladosporium dioscorae Haldar sp.nov., growing on the living...
Interval Appendectomy vs Conservative Management Alone - A Therapeutic Dilemma - A Retrospective Comparative study at Chennai Medical College Hospital and Research Centre - Irungalur, a Rural Tertiary Care Centre in South India
Background: Acute appendicitis is the most common cause of “acute abdomen.” In the 2 to 6 % of cases if not treated early it may develop in to an appendiceal mass. Conventionally conservative treatment followed by interv...
Stem Cells and Metallothionein - A Review
Stem Cells are capable of self renewal and can differentiate into other lineages upon induction with specific growth factors and proteins. Mesenchymal stem cells obtained from various sources like adipose tissue, umbilic...