Characterization of Amylase Inhibitor from the Seeds of Mucuna utilis
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2017, Vol 7, Issue 9
Abstract
Abstract: Proteinaceous amylase inhibitors are present in plants which regulate the activity of amylases. An α- Amylase inhibitor was isolated and purified by conventional protein purification techniques (ammonium sulphate fractionation, Sephadex G-10, sephadex G-50 chromatography and HPLC) from Mucuna utilis seeds. Its molecular weight as determined by gel-permeation chromatography on Sephadex G-100 was found to be 15 kDa. The purified inhibitor was temperature stable and retained more than 75% activity at 65 °C. Inhibitor was found to have pH optima of 6.9. 100% Zone of inhibition was observed when the inhibitor was added on the plated organisms. The Mucuna utilis amylase inhibitor was found to inhibit the activity of human salivary α- amylase. Inhibitory activity of α-amylase inhibitor against mammalian amylases could suggest its potential in treatment of diabetes and cure of nutritional problems, which result in obesity.
Authors and Affiliations
Rajiv Bharadwaj P, K S Chandrashekharaiah .
Keywords
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