Characterization of antimicrobial resistant bacterial pathogens recovered from cases of bovine mastitis with special reference to Staphylococcus aureus
Journal Title: UNKNOWN - Year 2016, Vol 23, Issue 1
Abstract
In the current study, a total of 20 and 78 milk samples were collected from animals showed signs of clinical and subclinical mastitis, for isolation and identification of different causative pathogens in some dairy farms of Beni-Suef Governorate, and for investigation of in vitro sensitivity. The recovered microorganisms were Staphylococcus species (n=79; 80.61%), Enterococcus spp. (n=28; 28.57%), CAMP negative Streptococci, Pseudomonas aeruginosa (n=7; 7.14%), E. coli (n=3; 3.06%) and Proteus vulgaris (n=1; 1.02%). Antibiogram profile for S. aureus showed that the most effective drug was vancomycin and the least was penicillin. Trials were done to detect biofilm production for recovered isolates of S. aureus (n=23) by the use of a phenotypic method (Congo red agar, CRA) and genotypic methods through determination of some biofilm related genes using PCR. All recovered S. aureus isolates were seeded on the CRA media to detect the biofilm forming ability. It has been found that all tested isolates showed a biofilm forming ability either strong (13; 56.52%) or intermediate (10; 43.48%). The detection of some biofilm associated genes (icaA, icaD and bap genes) using polymerase chain reaction revealed that two (10.53%) isolates out of 19 were negative for all tested genes, 16 (84.21%) isolates harbored both icaA and icaD gene, while only one (5.26%) isolate had all tested genes.
Authors and Affiliations
Hassan W. H. a,*, Hatem M. E. b, Elnwary H. A. c, Sediek S. H. c a Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt. b Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Cairo University, Egypt. c Animal Health Research Institute, Beni-Suef Branch, Beni-Suef, Egypt.
Keywords
Related Articles
- EP ID EP95068
- DOI 10.21608/jvmr.2016.43198
- Views 205
- Downloads 0
Bacteriological studies on bacterial pathogens isolated from broiler chickens with swollen head syndrome
This work was planned to investigate the bacteria isolated from broiler chickens head suffered from naked eye pathological lesions. Out of 200 examined head lesions, the result revealed that the major pathogens associ...
Vancomycin resistance among methicillin-resistant Staphylococcus aureus isolates from animal milk
Staphylococcus aureus(S. aureus) is a major cause of mastitis in dairy animals and a serious pathogen affecting human health. The current study was designed to investigate the extent of S.aureus in milk samples collect...
Prevalence of fungal pathogens in broiler chickens and their environment
Fungal diseases of poultry have become problematic as bacterial and viral diseases. This study was designed to investigate the prevalence of fungal agents in broiler chickens and their environment. The prevalence of fu...
Multi-drug Resistant Enterococcus faecalis isolated from animal and human sources
This work was planned to investigate the prevalence of antimicrobial resistance of Enterococcus faecalis isolated from animal and human sources. Ten isolates of E. faecalis recovered from urinary tract infections in hu...
Color Doppler ultrasound as an accurate and rapid tool for early pregnancy diagnosis in buffaloes
The objective was to determine the accuracy of color Doppler ultrasound for diagnosis of early pregnancy in buffaloes based on the evaluation of corpus luteum blood flow (CLBF) on days 20 and 21 after mating. Local Egy...