Characterization of Molecular Evolution in Multi-Drug Resistant of Mycobacterium tuberculosis by rpoB Gene in Patient with Active Pulmonary Tuberculosis from Iranian Isolates
Journal Title: International Journal of Biomedical Science (IJBS) - Year 2009, Vol 5, Issue 4
Abstract
This is the first genetic biodiversity study of Mycobacterium tuberculosis in Iran. Thus, we investigated the genetic patterns of strains isolated in the first survey of anti-tuberculosis drug-resistance by rpoB gene as part of the Global Project of Anti-tuberculosis Drug Resistance Surveillance (IAU, Iran). A 411-bp fragment of the rpoB gene, containing the sequence of the 81-bp rpoB fragment, was amplified by PCR and the rpoB gene fragments of tuberculosis strains were sequenced using the Amersham auto sequencer. For analysing tree evolution used method UPGMA and Neighbour-Joining. Clinical isolates (34/163) were analyzed by using sequencing gene rpoB and genotyped by program MEGA. The results were compared with the international database. Multi-drug resistant (MDR) was 14% in never treated patients and 8% in previously treated patients. Mutations in rpoB gene and katG genes were detected in 95% and 84% of the MDR strains, respectively. Two clusters were found to be identical by the four different analysis methods, presumably representing cases of recent transmission of MDR tuberculosis. The other strains are divided into 2 groups: group A – similar to the standard and Eastern strains (China, Taiwan) and group B – strains of another genotype. They are grouped separately on the dendrogram and became prevalent in Iran (they are called Iranian residential strains). This study gives a first overview of the M. tuberculosis strains circulating in Iran during the first survey of anti-tuberculosis drug-resistance. It may aid in the creation of a national database that will be a valuable support for further studies.
Authors and Affiliations
Saeed Bostanabad
Keywords
Related Articles
- EP ID EP85255
- DOI -
- Views 108
- Downloads 0
Comparison of Intraocular Pressure Fluctuations Measured by Goldmann Applanation Tonometer and Pulsatile Ocular Blood Flow Analyser
Evaluation of the effect of glaucoma medication is restricted by measurement of IOP as a dynamic physiological parameter. Present paper aimed to compare IOP fluctuations over time using Goldmann applanation tonometry (IO...
Retroperitoneal Hemorrhage from Adrenocortical Carcinoma as a Poor Prognostic Factor
In most patients, adrenocortical carcinoma is diagnosed at an advanced stage of the disease. A sudden retroperitoneal hemorrhage may sometimes be the first symptom of the adrenal cancer. We describe four patients with ad...
Free Radicals, Antioxidants in Disease and Health
Free radicals and oxidants play a dual role as both toxic and beneficial compounds, since they can be either harmful or helpful to the body. They are produced either from normal cell metabolisms in situ or from external...
Intraoperative Ethanol Treatment as an Adjuvant Therapy of Pterygium Excision
Recurrence of pterygium is the main concern for ophthalmic surgeons after the excision of pterygium. To evaluate the efficacy and safety of ethanol treatment during pterygium excision in preventing the recurrence of pter...
Analysis of peptidases in non-infected and Trypanosoma cruzi-infected mouse embryo hepatocyte cells
Cellular peptidase profiles from non-infected and Trypanosoma cruzi-infected hepatocyte cell cultures were characterized by substrate-SDS-PAGE containing different copolymerized proteins. A 100-kDa metallopeptidase had...