Characterization of Polish feline B. henselae isolates by multiple-locus tandem repeat analysis and pulse-field gel electrophoresis
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2012, Vol 19, Issue 1
Abstract
Knowledge about molecular epidemiology of [i]B. henselae[/i] is important for recognizing the geographical distribution of strains and identification of isolates virulent for humans. Eleven Polish feline [i]B. henselae[/i] isolates were typed, using 2 different techniques: pulse-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem repeat analysis (MLVA). PFGE analysis distinguished 6 different PFGE types, with subtypes within 3 of them, whereas 10 MLVA types were assigned. Global diversity index (D.I.) for MLVA equaled 0.93. For 7 isolates, the results of MLVA confirmed cluster assignments based on PFGE. Both PFGE and MLVA results were in accordance with epidemiological data. Although PFGE has been previously demonstrated to be a suitable method for the differentiation of [i]B. henselae[/i] isolates/strains, our results show the superiority of MLVA over PFGE with respect to higher discriminatory power, distinguishing genotypes I and II isolates, easier analysis of results, and possibility to compare the numerical data obtained by different laboratories. With MLVA, 7 new profiles were observed, compared to previous results from around the world; whereas 3 known profiles were previously described mainly in European [i]B. henselae[/i] isolates. Our results confirm that some VNTR profiles can be used as specific geographical markers.
Authors and Affiliations
Edyta Podsiadly, Nadia Haddad, Moez Berrich, Rim Bouchouicha, Benott Durand, Martine Monteil, Henri-Jean Boulouis, Stanislawa Tylewska-Wierzbanowska
Keywords
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