Characterization of Vaginal Lactobacillus Strains and Their Potential Antagonistic Effects on Candida albicans
Journal Title: Microbiology Research Journal International - Year 2015, Vol 6, Issue 4
Abstract
Aims: To characterize Lactobacillus strains for potential beneficial effects, especially for inhibition of Candida albicans growth. Materials and Methods: Twelve vaginal Lactobacillus strains belonging to five species from our laboratory collection were studied. The acid production and hydrogen peroxide (H2O2) production were determined. The biofilm formation ability was measured by a semi-quantitative microtiter plate assay. Besides the detection of autoaggregation and coaggregation capacities with C. albicans using a spectrophotometric assay, aggregating clusters were observed by light microscopy. The spot inhibition, agar overlay and Oxford Cup methods were used to test antagonistic activities of Lactobacillus strains against C. albicans. Results: Seven strains of L. fermentum (A011, A024, A025, A035, A041, A044 and A081) and L. salivarius A023 were revealed to decrease the medium pH below 4.5 just within six hours due to the rapid growth. L. jensenii A083 and L. gasseri A054 were the best biofilm formers in polystyrene plates, whereas the other ten strains could form biofilm with varying affinities. In addition to significant H2O2 production found in L. jensenii A083, L. crispatus A014 and A055, these strains showed strong autoaggregation and coaggregation abilities with C. albicans (ATCC 90029 and Z03). L. salivarius A023, L. fermentum A025 and L. crispatus A014 displayed strong activities against C. albicans in spot inhibition and agar overlay assays. Conclusion: The potential beneficial properties of Lactobacillus spp. were strain specific. Among the tested strains, L. salivarius A023, L. fermentum A025 and L. crispatus A014 might be better candidates for further investigation on the inhibition of C. albicans growth.
Authors and Affiliations
Shuai Wang, Qiangyi Wang, Bingbing Xiao, Rui Zhang, Ben Wang, Qinping Liao, Hui Zhuang, Tong Li
Keywords
Related Articles
- EP ID EP355000
- DOI 10.9734/BMRJ/2015/15116
- Views 85
- Downloads 0
Biosorption of Heavy Metals in Dumpsite Leachate by Metal-resistant Bacteria Isolated from Abule-egba Dumpsite, Lagos State, Nigeria
Aim: We studied the potentials of four metal-resistant bacterial strains to reduce the concentration of selected metals in dumpsite leachate. Methods: The bacteria which were obtained from the culture collection of the E...
Bacterial and Drug Susceptibility Profiles of Urinary Tract Infection in Diabetes Mellitus Patients at Mbarara Regional Referral Hospital, Uganda
Aim: The risk of developing infection in diabetic mellitus patients is known to be higher than in normal individuals. The urinary tract is the most common entry point of infections. Surveillance of urinary tract pathogen...
Investigation of Astaxanthin Production from Yeast Rhodosporidium sp.
Aims: Astaxanthin, especially natural astaxanthin, is a powerful antioxidant, which is used as a nutraceutical and a common coloring agent in aquaculture. The present study was carried out to investigate the ability of a...
Antibiotic Resistance Patterns of Lactic Acid Bacteria Isolated From Different Fermented Milk Products of Turkish Origin
Aims: Lactic acid bacteria isolated from 48 Turkish fermented milk products obtained from local markets, dairies or bazaars were investigated for their resistance of antibiotics including ampicillin, erythromycin, gentam...
Role of Human Papillomavirus Infection in the Development of Prostate Carcinoma and Benign Prostatic Hyperplasia
Aims: Human papillomavirus (HPV) infections are associated with benign and malignant lesions of the female and male anogenital tract. Currently, the possible role of HPV infections in prostate carcinogenesis is a subject...