Checking the Plentitude of (BLA-SHV) ß-lactamase Gene in Separated E. Colis in Children under 6 Years-Old Infected with Urinary Tract Infection Referring to Tehran Central Pathobiology Laboratory (2014)
Journal Title: European Online Journal of Natural and Social Sciences - Year 2017, Vol 6, Issue 3
Abstract
Increasing consumption of Antibiotics like β-lactam antibiotics including Penicillins and Cephalosporins has resulted in the creation of refractory E. coli levels to these antibiotics. The aim of this study is investigating the presence of resistant gene to β-lactam antibiotics and third-generation Cephalosporins among separated E. coli levels from urine sample of the referring children under 6 infected with urinary tract infection to Tehran central pathobiology laboratory. 130 s E.coli sample were collected from urine sample of the children. Sensitivity of the collected levels to β-lactam antibiotics including Ampicillin, Amoxicillin and third generation Cephalosporins was measured. Also, PCR was implemented for finding BLA-SHV gen. Among 130 samples under study, 45 samples comported BLA-SHV gen. 100 samples (77%) were resistant toward Amoxicillin and Ampicillin antibiotics and 30 samples (23%) were sensitive to these antibiotics. In this study, resistant percentage rate to non-ß-lactam antibiotics like Amikacin, Gentamicin, Cotrimoxazole, and Ciprofloxacin was respectively 0%, 14%, 66% and 23%. This study has shown the presence of BLA-SHV gen with an abundance of 35% in E. coli bacteria and it also confirms the importance of awareness of growing increase of Antibiotic resistance and reduction and change in antibiotics’ consumption strategy.
Authors and Affiliations
Sohrab Ahrabi, Jalaluddin Derakhshanpour Khamene
Keywords
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