Cholera toxin A1 residues single alanine substitutional mutation and effect on activity with stimulatory G protein
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 6
Abstract
Cholera is a well-known gastrointestinal infection. The cholera toxin is an important pathological substance in pathogenesis of cholera diarrhea. Cholera toxin is composed of catalytic A1 subunit, an A2 linker, and a homopentameric cell-binding B subunit. In enterocyte, cholera toxin will attach to GM1 ganglioside receptors on the apical membrane and causes retrograde vesicular trafficking to endoplasmic reticulum. At endoplasmic reticulum, cholera toxin A1 is released from the rest of the toxin into cytoplasm. The cholera toxin A1 interacts will catalyze ADP ribosylation of subunits of stimulatory G protein resulting a persistent activation of adenylate cyclase and an elevation of intracellular cAMP which further result in diarrhea. The single alanine substitutional mutation can result in the reduction of the interaction activity between cholera toxin A1 and stimulatory G protein. In this study, the four well-known mutations, H55, R67, L71, S78, or D109, of cholera toxin A1 is focused. The author hereby calculates for the reaction energy for the reaction between cholera toxin A1 and stimulatory G protein in na¨ıve case and mutated case. To calculate, the standard bonding energy calculation technique in mutation analysis was used. It can be seen that aberrant in reaction energy in each studied mutation is different and can imply the different effect on activity with stimulatory G protein.
Authors and Affiliations
Somsri Wiwanitkit, Viroj Wiwanitkit
Keywords
Related Articles
- EP ID EP254516
- DOI 10.1016/j.apjtb.2017.05.012
- Views 52
- Downloads 0
Cytotoxic, apoptotic and cell migration inhibitory effects of atranorin on SPC212 mesothelioma cells
Objective: To investigate the effects of atranorin, a lichen secondary metabolite, on SPC212 malignant mesothelioma cells in vitro. Methods: SPC212 malignant mesothelioma cell line was used. 3-(4,5-dimethylthiazol-2-yl)-...
Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack
Objective: To examine the in vitro and in vivo anti-inflammatory effects of the alkaloid enriched extract (ELA) from the roots of Eurycoma longifolia. Methods: The in vitro antiinflammatory effects of ELA were evaluated...
Phytochemical investigation, antioxidant and wound healing activities of Citrullus colocynthis (bitter apple)
Objective: To undertake metabolite profiling of various plant parts of Citrullus colocynthis, and assess antioxidant and wound healing activities of fractions for therapeutical applications. Methods: Extracts from leaves...
Chemical constituents, in vitro antimicrobial and cytotoxic potentials of the extracts from Macaranga barteri Mull-Arg
Objectives: To investigate antimicrobial and cytotoxic potentials as well as chemical constituents of extracts from Macaranga barteri (M. barteri). Methods: Antimicrobial activity was carried out using micro-dilution, ce...
Optimization of ionic liquid-based microwave-assisted extraction of polyphenolic content from Peperomia pellucida (L) kunth using response surface methodology
Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content from Peperomia pellucida (L) Kunth. Methods: The IL-MAE factors as experimental design parameters, including mi...