CHOLESTEROL LOWERING POTENTIALS OF A BLEND OF STANDARDIZED METHANOL EXTRACTS OF MORINGA OLEIFERA LEAVES AND FRUITS IN ALBINO WISTAR RATS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 11
Abstract
Objective: Moringa oleifera Lam. (Moringaceae), a small rapid growing, evergreen, deciduous tree is an important medicinal plant. Leaves and fruits of this plant are used for various ailments, as a nutritional supplement and also as vegetables. The current study involves in the determination of best combination of the cholesterol-lowering potential of a blend of methanol extracts of M. oleifera leaf and fruits, developed based on in vitro FIC index studies and evaluate the combination of this extracts in hypercholesterolemic animal models.Methods: Leaf and fruit methanol extracts and their combinations were tested in in vitro lipase inhibition assay to determine the best combination using fractional inhibitory concentration (FIC) index. Hypercholesterolemia was induced with Triton WR-1339 (a non-ionic detergent) and with high cholesterol diet for acute and chronic model respectively and the cholesterol-lowering effect of 1:1 blend of M. oleifera leaf and fruits methanol extracts was evaluated.Results: The FIC index values indicated that M. oleifera leaf and fruit extracts blended in 1:1 proportion was the best combination in in vitro lipase inhibition assay. This blend, when evaluated in vivo, showed a significant decrease in serum total cholesterol level from 24 h through 48 h in triton model. In high cholesterol diet model, the extract blend showed a significant reduction in serum triglycerides levels at 3 and 6 w of treatment.Conclusion: The results indicate that the blend of M. oleifera at the tested dose could be lowering cholesterol and triglyceride levels by inhibiting the absorption of cholesterol and can be developed as a standardized blend for dietary supplement market.
Authors and Affiliations
Gururaja G. M. , Deepak Mundkinajeddu, Senthil Kumar A. , Joshua Allan J. , Shekhar M. Dethe, Amit Agarwal
Keywords
Related Articles
- EP ID EP575918
- DOI 10.22159/ijpps.2016v8i11.14014
- Views 77
- Downloads 0
DEVELOPMENT OF STABILITY INDICATING RP-HPLC METHOD FOR THE DETERMINATION OF RELATED SUBSTANCES IN ATORVASTATIN SOLID DOSAGE FORM AND BULK DRUGS
Objective: The present paper reports the development of simple, rapid, accurate and stability indicating reversed phase high-performance liquid chromatography method (RP-HPLC) for estimation of related substances in Ator...
EFFECT OF MOMORDICA CHARANTIA AND SYZYGIUM CUMINI EXTRACT ON SERUM ELECTROLYTES IN ALLOXAN INDUCED DIABETIC RATS
Objective: Diabetes is a group of disorders characterized by high blood glucose levels. Disturbances in serum electrolytes like sodium (Na+) and potassium (K+) are found in diabetes. The purpose of the study was to inves...
FACILE SYNTHESIS, SPECTRAL STUDIES, DFT CALCULATIONS AND BIOLOGICAL ACTIVITIES OF NOVEL NI (II), CU (II), AND PD (II) COMPLEXES OF THIADIAZOLE ANALOGS
Objective: A facile synthesis of some novel Schiff base derivatives of 2-substituted-5-amino-thiadiazoles along with their Ni (II), Cu (II), and Pd (II) complexes were achieved by sonication and the conventional method....
DIFFERENTIAL EXPRESSION OF NITRIC OXIDE SYNTHASES IN THE SKIN TISSUE OF P-PHENYLENEDIAMINE-TREATED MICE
Objective: p-Phenylenediamine (PPD) is a possible contact sensitizer in skin tissues; however, data are lacking regarding its specific effects on nitric oxide synthase (NOS) expression during the sensitization phase. The...
THE EFFECTS OF COSMOS CAUDATUS AND PIPER SARMENTOSUM AQUEOUS EXTRACTS ON MALE MICE FERTILITY
Objective: This study was conducted to determine the sperm quality and fertility of male mice supplemented with Cosmos caudatus and Piper sarmentosum aqueous extracts.Methods: The male mice were treated with either 0.2 m...