CHROMATOGRAPHIC ISOLATION OF ALLIUM CEPA (SSP. RED ONION) AND ITS CYTOTOXIC ACTIVITY AGAINST HUMAN LIVER CARCINOMA CELL LINES (HEPG2)
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: The present study aimed to find out cytotoxic agent from Allium cepa L. (ssp. red onion) family Alliaceae as well as chromatographic isolation of its active constituents.Methods: The defatted methanol extract of Allium cepa (ssp. red onion) was fractionated with chloroform, ethyl acetate and n-butanol. Each of ethyl acetate and butanolic fraction was separately submitted to chromatographic isolation using different chromatographic techniques such as column chromatography (CC), thin layer chromatography (TLC) and paper chromatography (PC). The structures of the isolated compounds were elucidated through spectroscopic analysis.Results: Four compounds were isolated from ethyl acetate fraction and identified as kaempferol (1), quercetin (2) isorhamnetin (3) and isorhamentin-4`-O-β- D-glucopyranoside (4) whereas two compounds were isolated and identified from the butanolic fraction as α -D-glucopyranose (5), β-D-glucopyranosyl- α- D-glucopyranoside (6). The cytotoxic activity of defatted methanolic extract of A. cepa (ssp red onion), ethyl acetate and butanolic fractions against human liver carcinoma cell line (HepG2) was IC50 = 10.9, 6.08 and 9.95 µg/ ml respectively. Also, compounds 4 isolated from ethyl acetate fraction and compound 6 isolated from butanolic fraction showed cytotoxic activity (IC50 = 11.90 and 22.0 µg/ ml) respectively.Conclusion: It was appeared that the ethyl acetate fraction was the most active due to it contain high amount of phenolic compounds.Â
Authors and Affiliations
Mahfouz M Abdel-gawad, Maher A El-hashash, Mortada M El-sayed, Eman A El-wakil, Ezzat El-sayed Abdel-lateef
Keywords
Related Articles
- EP ID EP579669
- DOI -
- Views 55
- Downloads 0
DISCRIMINANT ANALYSIS OF PURIFICATION ON TURMERIC (CURCUMA LONGA LINN) SAMPLES BASED ON PHYTO-PHARMACOGNOSTICAL AND MULTIVARIATE CHEMOMETRIC TECHNIQUE
Objective: To establish an acceptable identification system of various purification effects in context classical based different media on turmeric samples and relates its altering pattern in favor of phyto-pharmacognosti...
CHEMO-PROTECTIVE EFFECT ON HEPATO-RENAL TOXCICITY AND CYTOTOXIC ACTIVITY OF LIPOIDAL MATTER OF ATRIPLEX LINDLEYI MOQ
Objective: Bromobenzene (BB) is frequently encountered in table-ready foods as contaminant residues. Therefore, the present study is designed to evaluate the petroleum ether extract of Atriplex lindleyi to attenuate the...
ANTIMICROBIAL POTENTIALOF HEMOLYMPH AND HEPATOPANCREAS OFPORTUNUSSEGNISCRABS
Objective: The potential of marine crabs as a source of bioactive products is largely unexplored. Methods: Portunussegnis crabs were collected from the northern coast of Persian Gulf. Antimicrobial activities of hepatopa...
DETECTION AND CHARACTERIZATION OF ENTEROCIN ENCODING GENES IN ENTEROCOCCUS MUNDTII STRAIN C4L10 FROM THE CECUM OF NON-BROILER CHICKEN
Objective: This study was carried out to isolate and detect the enterocin genes of the bacteriocin producing strain Enterococcus mundtii C4L10 isolated from Malaysian non-broiler chicken.Methods: Amplified bacteriocin en...
IN VIVO ANTICANCER EVALUATION OF (2-AMINO-3,4,5-TRIMETHOXYPHENYL) (6-METHOXY-1H-INDOL-3-YL) METHANONE
Objective: The design and development of Combretastatin A-4 analogues as anticancer agent has always attracted the attention of scientific community involved in anticancer research. The aim of the present work was to inv...