Chronic Effects of Maternal Malnutrition during Lactation: Severe Influences on Cortical Bone Mass of Wistar Rats Femur
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 11
Abstract
Introduction: Malnutrition is a world health problem and has high incidences in developing countries. It is known that the nutritional status of the mother during gestation and lactation affects the growth and development of its child. Maternal malnutrition during breastfeeding has been shown to cause alterations of thyroid function and bone tissue. Aim: The present study aimed to evaluate the chronic effects in bone tissue of energy-protein restriction in rats during lactation period through a histological and morphometric analysis of the cortical mass of the femur. Materials and Methods: At parturition, Wistar rat dams were divided in three groups: (1) Control group - which received 23% protein diet without restrictions; (2) Protein-Energy restriction group - which received 8% protein diet; (3) Energy restriction group - which received 23% protein diet in limited amounts, according to the ingestion of the second group. Each group had 12 pups. After weaning, all pups were separated from its dams and received free access to 23% protein diet until 180 days and then euthanized. Afterwards, the femur of these specimens were excised and fixed in 4% formaldehyde solution and decalcified by EDTA. 10 µm thick transverse sections on the middle third of the femur diaphysis were performed. Then, the tissue was stained with Haematoxylin/Eosin and analysed under a microscope. Results: The cortical area of the control, energy restriction and protein-energy restriction groups were, respectively: 6.54±0.23 µm, 5.51±0.29 µm and 6.18±0.19 µm, while the medullary cavity showed the following results: 3.65±0.16 µm, 4.1±0.20 µm and 3.53±0.12 µm. Reduction of the cortical area and an increase of medullary zone were observed on the protein-energy restriction group. Conclusion: Maternal malnutrition during lactation has negative effects on femur growth even after normalization of the diet in adult aged Wistar rats.
Authors and Affiliations
Guilherme Schultz, Fabiano Medeiro, Mauricio Arcoverde, Max Alves Cavalcante, Lucas Pires, Monique Babinski, Marcio Antonio Babinski
Keywords
Related Articles
- EP ID EP366834
- DOI 10.7860/JCDR/2017/29004.10874
- Views 63
- Downloads 0
Revisiting the Utility of Histopathological Examination of Biopsy: A Necessity in Microbiology
Introduction: Culture is the gold standard, while potassium hydroxide mount is simplest technique used for diagnosis of fungal pathogens. Histopathological examination is the only definitive means to identify certain unc...
Importance of Ayu Pareeksha for the Management of Diseases
The word Ayurveda comes from the Sanskrit root Ayu which means span of life. The purpose of examination of Ayu is to obtain knowledge regarding longevity, residual span of life in diseased person and rate of the mortalit...
Effect of Cellular Mobile Phone Use and Cetrizine on Hand-Eye Coordination and Visual Acuity
Introduction: Cellular mobile phones are a major cause of distraction especially while driving. The aggressive and inappropriate use of cellular mobile phones has increased the risk of accidents. Similar alerts are avail...
Clinical Profile of Haemorrhoid Cases Admitted in Various Tertiary Care Hospitals in an Urban Area of Southern India
Introduction: Clinical presentation of haemorrhoids is varied resembling number of anorectal diseases. Aim: To assess risk factors, clinical profile and management practices of haemorrhoid cases. Materials and Methods: T...
Knowledge, Attitude and Practices regarding Dengue among Outpatients and their Attenders in Three Primary Health Care Centres in Coimbatore, Tamil Nadu, India
ABSTRACT Introduction: Dengue is an arboviral disease transmitted by Aedes aegypti Mosquitoes with incidence of 50 to 100 million infections annually worldwide. Since no effective vaccine for Dengue is commercially avail...