Classifying Ethiopian Tetraploid Wheat (Triticum turgidum L.) Landraces by Combined Analysis of Molecular & Phenotypic Data
Journal Title: STAR Journal - Year 2012, Vol 1, Issue 1
Abstract
The aim of the study was to investigate the extent of the genetic diversity among gene bank accessions of Ethiopian tetraploid wheat (Triticum turgidum L.) landraces using microsatellite markers, qualitative and quantitative data. Thirty five accessions of Ethiopian tetraploid wheat (T. turgidum L.) landraces were grown in the greenhouse at IFA Tulln, Austria during spring 2009 for DNA extraction. The same accessions were already grown in spring 2008 at BOKU Vienna, Austraia for their phenotypical characterisation. DNA was extracted from each approximately one month old plant according to Promega (1998/99) protocol. A total of 10 µl reaction mixture per sample was used for DNA amplification by PCR. The amplified mixture was loaded to PAGE (12%) containing TE buffer (1) in CBS electrophoresis chambers and run in an electric field for 2 hrs. The fragments were visualized by scanning with Typhoon Trio scanner. Six and ten quantitative and qualitative morphological traits data respectively were used for combined analysis. Genetic variation was significant within and between wheat species and within and between altitudes of collection site. Genetic distances ranged from 0.21 to 0.73 for all accessions while it ranged from 0.44 within Triticum polonicum to 0.56 between T. polonicum and T. turgidum. Genetic distance between regions of collection ranged from 0.51 to 0.54 while for altitudes it ranged from 0.47 (≤2200 m) to 0.56 (≤2500 m). Cluster analysis showed that T. polonicum accessions were grouped together whereas T. durum and T. turgidum formed mixed clusters indicating T. polonicum as genetically more distinct from the other two species. We suggest combined analysis of molecular and morphological data for a better classification of accessions.
Authors and Affiliations
Negash Geleta| Department of Plant Sciences, Wollega University, Post Box No: 395, Nekemte, Ethiopia, Heinrich Grausgruber | BOKU- University of Natural Resources and Applied Life Sciences, Department of Applied Plant Sciences and Plant Biotechnology, Institute of Agronomy and Plant Breeding, Vienna, Austria, A-1180
Keywords
Related Articles
- EP ID EP9445
- DOI http://dx.doi.org/10.4314%2Fstar.v1i1.98767
- Views 537
- Downloads 25
Impacts of Land use on Selected Physicochemical Properties of Soils of Gindeberet Area, Western Oromia, Ethiopia
This study was carried out with the objective of determining impact of land uses on selected soil physicochemical properties of Gindeberet area, Western Ethiopia. Disturbed and undisturbed surface soil samples (0-20 cm...
Knowledge, Perception and Practice of Mothers/Caretakers and Family’s regarding Child Nutrition (under 5 years of age) in Nekemte Town, Ethiopia
The purpose of this study was to assess the knowledge, perception and practices of mothers/caretakers, families and community regarding child nutrition. A community based descriptive cross-sectional study was employed...
Genetic Diversity of Local and Introduced Sweet Potato [Ipomoea batatas (L.) Lam.] Collections for Agro-morphology and Physicochemical Attributes in Ethiopia
Efficient use of plant genetic diversity is a prerequisite for the development of crop varieties in meeting the challenges of food security and poverty alleviation. Understanding the variation and diversity of the geneti...
Anticariogenic Activity of Lagerstroemia speciosa (L.)
Dental caries is the common infectious diseases of the oral cavity and is caused mainly by oral streptococci. The present study was carried out to investigate the anticariogenic activity of methanol extract of Lagerstr...
Effects of Different Pasteurizers on the Nutritional Quality of Raw Milk Samples
No Abstract