Clinicomycological Study of Otomycosis with Antifungal Susceptibility Testing Of Fungal Isolates.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2019, Vol 18, Issue 2
Abstract
Otomycosis or fungal otitis externa is a superficial, sub-acute or chronic infection of the external auditory canal, usually with unilateral presentation characterized by inflammation, pruritis, scaling and otalgia. Aim Of The Study: To find the prevalence of otomycosis along with its clinicomycologicalprofile,to explore specific predisposing factors,to isolate and identify the causative fungus and antifungal susceptibility testing for moulds by E test and for yeast by disk diffusion method. Methodology:Ear swabs were collected from 30 clinically suspected cases of otomycosis and all the samples were processed by direct microscopy of KOH mount and Gram staining. Culture was carried out on SDA.The identification process of the isolated fungus was done by standard procedures. Antifungal sensitivity of moulds was performed on RPMI 1640 agar supplemented with 2% glucose and tested for fluconazole (0.016-256 mcg/ml), Itraconazole(0.002 mcg/ml to 32 mcg/ml) and Caspofungin (0.002-32 mcg/ml) (HiMedia) by E strip .AFST for yeast was performed by the disc diffusion method on Mueller Hinton Agar supplemented with 2% glucose and 0.5mg/ L Methylene Blue (HiMedia)and tested for Fluconazole (10μg),Nystatin (100U), Amphotericin (100U), Ketoconazole (10μg) and Clotrimazole (10μg) disk (HiMedia). Result And Conclusion:Otomycosis was diagnosed in 100% of the cases with highest prevalence in 21-40 years of age group with a female preponderance.Itching was found to be the commonest symptom followed by ear pain.Ear pricking with hard objects was found to be the most common predisposing factor followed by swimming or pond bath. Seven cases were associated with tympanic membrane perforation. Of the 30 cases 32 number of fungi were isolated. Fungal isolates were predominantly mould (90.62%) while rest were yeast (9.38%). Mixed infection with more than one fungus was seen in 2 cases (6.67%). A.niger was the predominant fungus (71.88%) followed by A.flavus(18.75%) and C.tropicalis(9.37%). All the yeasts were found to be sensitive to Ketoconazole,Nystatin and Amphotericin and all moulds were found to be resistant to fluconazole.
Authors and Affiliations
Monalisa Panigrahi, BimochProjna Paty, Banojini Parida, Sanghamitra Padhi, Susmita Kumari Sahu, M. V. Narasimham, Indrani Mohanty
Keywords
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- EP ID EP467671
- DOI 10.9790/0853-1802130712.
- Views 70
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