Cloning and Characterization of a Human Novel Gene Netrin-G2
Journal Title: Journal of Neurological Sciences-Turkish - Year 2008, Vol 25, Issue 2
Abstract
The Netrins is one of a small phylogenetically conserved family members which are laminin- related secreted proteins. The Netrins play a critical role in cell migration and axon guidance. Its homologues have been widely described in various animals. Netrins can be subdivided into two subfamilies, classical Netrins and Netrin-Gs. Since the members of Netrin-G subfamily exhibit a high degree of similarity and there is only one member, Netrin-G1, identified in human, it is presumed that there must be new members in human too. In this paper the total RNA in brains was extracted from 20 weeks human embryos, and the cDNA Library was constructed by using a cDNA PCR Library Kit. A full-length human netrin-G cDNA, named netrin-G2, was generated by using special primers and PCRs. Finally, a primary study about the expression of human netrin-G2 was carried out by northern blot analysis and a research about the relationship between human Netrin-G2 and other Netrins family members was determined by phylogenetic tree analysis. Human netrin-G2 was indeed a new member of netrin-G subfamily, which mapped to chromosome 9q34. The final assembled cDNA sequence of human netrin-G2 was 2428 bps in length, which could encoding a 530-amino acid protein. Northern blot analysis revealed that netrin-G2 expressed specifically in brain and seldom in other tissues. These results suggested that netrin-G2 might play an important role in the development of central nervous system and might contribute to excitatory neurotransmission and neural modulation.
Authors and Affiliations
Ye XIANGLI, Lei LIFANG
Keywords
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