Cloning, expression, and enzymatic activity analysis of strawberry S-adenosyl-L-metnionine synthetase gene FaSAMS1
Journal Title: JOURNAL OF ADVANCES IN AGRICULTURE - Year 2016, Vol 6, Issue 1
Abstract
The S-adenosyl methionine synthetase gene FaSAMS1 is involved in the regulation of strawberry fruit ripening, however, the biochemical properties of FaSAMS1 protein remain unclear. Here, a coding cDNA sequence of FaSAMS1 was cloned by RT-PCR and inserted into a recombinant yeast expression vector pPICZA, then transformed into the yeast expression strain X-33. The fusion protein FaSAMS1 was induced, expressed, and purified. The enzymatic activity analysis of FaSAMS1 showed that the reaction system contains 0.09 mg of FaSAMS1, protein concentration reached at 0.454 mg·mL-1, the activity of the enzyme was 0.32 U, specific activity was 3.56 U·mg-1. This study has provided biochemical evidence for the involvement of ethylene in the regulation of strawberry fruit ripening.Â
Authors and Affiliations
Shu-Fang Wang, Li-Na Hua, Xiaofeng Liu, Yuanyue Shen
Keywords
Related Articles
- EP ID EP652407
- DOI 10.24297/jaa.v6i1.5390
- Views 227
- Downloads 0
Effects of water stress on the physiological growth indices on performance of soybean genotypes
Pot experiment was conducted at the Department of Agronomy Crop Pavilion of the University of Ilorin, Ilorinto assess the effects of water stress at different growth stages on the physiological parameters of soybean geno...
RESILIENCE IN DAIRY FARM BUSINESSES; TO BOUNCE WITHOUT BREAKING
New Zealand dairy farmers face an increasingly turbulent business environment. To cope with a turbulent environment, they need to have resilient farming systems that have the capacity to better deal with volatility. The...
COST OF MILK PRODUCTION AND BREAK EVEN ANALYSIS OF MEMBER AND NON MEMBER OF DAIRY COOPERATIVE SOCIETY FOR MILCH ANIMALS (COW & BUFFALO) IN DISTRICT ETAWAH OF U.P.
This study covered 150 cooperative member milk producers and 150 non-member milk producers which were post- stratified into Landless, Marginal, small, medium and large herd size categories. Breakeven point is a point whe...
Response of different bean genotypes to soil nutrients and water in the semi-arid areas of eastern Kenya
Beans (Phaseolus vulgaris L.) are important legumes in the semi-arid areas of Eastern Kenya. But their production is constrained by water stress within the season and between seasons. A field trial was conducted in the s...
A study on the contamination routes of tomato and bell pepper plants by Escherichia coli and Listeria innocua
Tomatoes and bell peppers have been the source of recent outbreaks of foodborne illnesses due to contamination by human pathogens such as E. coli O157:H7 and Listeria monocytogenesin the field. The objectives of the pres...