Cluster of Differentiation 45: An Adjunct to Flowcytometric Diagnosis of Leukemias
Journal Title: Iranian Journal of Blood and Cancer - Year 2013, Vol 5, Issue 3
Abstract
Background: This Cross sectional comparative study was performed to compare the mean fluorescence intensity of cluster of differentiation 45 between healthy individuals and patients with acute lymphoblastic leukemia. Patients and methods: Thirty three healthy individuals (mean age 11 years) and 41 patients with B cell acute lymphoblastic leukemia (mean age 7 years) were enrolled in the study in department of Immunology, Armed Forces Institute of Pathology, Rawalpindi, from Jan 2009 to April 2011, after ethics committee approval and obtaining informed consent. For immunophenotyping analysis, blood was stained with monoclonal antibodies using lyse wash procedure. For each subject, panel for staining consisted of cluster of differentiation 3, cluster of differentiation 5, cluster of differentiation 7, cluster of differentiation 10, cluster of differentiation 19, cluster of differentiation 20, , cluster of differentiation 13, cluster of differentiation 34, cluster of differentiation 1314, cluster of differentiation 1333, cluster of differentiation 45, HLA-DR, and intracytoplasmic myeloperoxidase as well as terminal deoxynucleotidyl transferase . Cells were then analyzed on Becton Dickinson FACSCalibur flow cytometer using Cell Quest Pro software. For each subject, mean fluorescence intensity was calculated in software along with geometric mean and standard deviation. Results: Mean of geometric means of cluster of differentiation 45 expression on blasts of patient population was considerably low (145) as compared to lymphocytes of healthy population (764), being statistically significant (p<0.0001). Conclusion: cluster of differentiation 45 is useful in differentiating mature lymphocytes from blasts in ALL cases. Keywords: Flow cytometry, acute lymphoblastic leukemia, cluster of differentiation 45, mean fluorescence intensity.
Authors and Affiliations
HN. Tipu
Keywords
Related Articles
- EP ID EP365839
- DOI -
- Views 102
- Downloads 0
The Comparison of Efficacy of Original Brand Deferoxamine with Generic Iranian Made Deferoxamine in Urinary Iron Excretion in Patients with Thalassemia Major
Background: Deferoxamine mesylate is still the conventional and well-known iron chelator for patients with thalassemia major. However, due to some marketing issues the well-known original brand, Desferal®, produced by No...
Central Hypothyroidism: A Rare Complication in a Child Undergoing Chemotherapy for Acute Lymphoblastic Leukemia
Abnormalities of the thyroid gland are among the most frequent endocrine complications observed in childhood cancer survivors. We report an 11 years old girl who developed central hypothyroidism and ACTH deficiency durin...
The Difference in Initial Leukocyte Count, Bone Marrow Blast Cell Count and CD 34 Expression in Patients with Acute Myeloid Leukemia with and without NPM1 gene Mutation
Background: Mutation in NPM1 gene has been reported to be the most common genetic mutation in de novo acute myeloid leukemia (AML). AML with NPM1 gene mutation usually presents with higher initial leukocyte and blast cel...
Invasive Aspergillosis in Pediatric Hematology Oncology Ward
Background: Patients with prolonged neutropenia and/or severe underlying immunosuppression are at the greatest risk for disseminated aspergillosis. This study was undertaken to determine the incidence of invasive aspergi...
Zinc Supplementation Effect on Linear Growth in Transfusion Dependent β Thalassemia
Objective: Thalassemic patients are at risk of zinc deficiency due to various causes including desferal injection, hyperzincuria, high ferritin levels, and hepatic iron overload. We evaluate the effect of zinc supplement...